Development of multidimensional liquid chromatography and application in proteomic analysis

被引：18

作者：

Gao, Mingxia ^{[1
]}

Qi, Dawei ^{[1
]}

Zhang, Peng ^{[1
]}

Deng, Chunhui ^{[1
]}

Zhang, Xiangmin ^{[1
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

来源：

EXPERT REVIEW OF PROTEOMICS | 2010年 / 7卷 / 05期

基金：

中国国家自然科学基金;

关键词：

magnetic nanoparticles; multidimensional liquid chromatography; post-translational modifications; proteomic analysis; quantitative analysis; TANDEM MASS-SPECTROMETRY; PROTEIN-IDENTIFICATION TECHNOLOGY; HYDROPHILIC INTERACTION CHROMATOGRAPHY; N-LINKED GLYCOPROTEINS; NECK CANCER BIOMARKERS; HIGH SEQUENCE COVERAGE; CAPILLARY TRAP COLUMN; LARGE-SCALE ANALYSIS; HUMAN LIVER-TISSUE; REVERSED-PHASE;

D O I：

10.1586/EPR.10.49

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

As a complementary approach to 2D-PAGE, multidimensional liquid chromatography (MDLC) separation methods have been widely applied in all kinds of biological sample investigations. MDLC coupled with mass spectrometry is playing an important role in proteome research owing to its high speed, high resolution and high sensitivity. Among MDLC strategies, ion-exchange chromatography together with reversed-phase LC is still a most widely used chromatography in proteome analysis; other chromatographic methods are also frequently used in protein prefractionations. Recent MDLC technologies and applications to a variety of proteome analyses have achieved great development. The diversity of combinations of different chromatography modes to set up MDLC systems was demonstrated and discussed. Novel developments of MDLC techniques such as ultra-pressure system, array-based separation and monolithic material are also included in this article.

引用

页码：665 / 678

页数：14

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