The protective effect of thymoquinone on tert-butylhydroquinone induced cytotoxicity in human umbilical vein endothelial cells

被引：8
作者：
Karimi, Zahra [1 ,2 ,3 ]
Ghaffari, Maryam [1 ]
Dolatabadi, Jafar Ezzati Nazhad [4 ]
Dehghan, Parvin [2 ]
机构：
[1] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran
[2] Tabriz Univ Med Sci, Fac Nutr & Food Sci, Nutr Res Ctr, Dept Food Sci & Technol, Tabriz, Iran
[3] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran
[4] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran
来源：
TOXICOLOGY RESEARCH | 2019年 / 8卷 / 06期
关键词：
NIGELLA-SATIVA; APOPTOSIS; TBHQ; GENOTOXICITY; DAMAGE;
D O I：
10.1039/c9tx00235a
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
2-tert-Butyl-4-hydroquinone (TBHQ) is used for inhibition of oxidative rancidity in the food industry. However, this antioxidant can stimulate cytotoxicity in human umbilical vein endothelial cells (HUVECs). Thus, potential protective effects of thymoquinone (TQ) against TBHQ-induced cytotoxicity were investigated. Cytotoxicity was evaluated via MTT, flow cytometry, DAPI staining and DNA fragmentation methods. The obtained results revealed that treatment of HUVECs with TQ enhanced the cell viability rate and it had potential to reduce the cytotoxicity effect of TBHQ in cells. Also, in a combined regime of TQ and TBHQ, apoptosis was reduced compared to the cells treated with TBHQ (p < 0.05). Similarly, TQ had a protective effect on DNA and chromatin fragmentation of the cells treated with TBHQ. Finally, it can be concluded that TQ could be used as a protective agent against cytotoxicity induced by TBHQ in HUVECs.
引用
收藏
页码：1050 / 1056
页数：7
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