Characterization of novel microsatellite markers in the Antarctic silverfish Pleuragramma antarcticum and cross species amplification in other Notothenioidei

被引：3

作者：

Papetti, Chiara ^{[1
]}

Marino, Ilaria Anna Maria ^{[1
]}

Agostini, Cecilia ^{[1
]}

Bisol, Paolo Maria ^{[1
]}

Patarnello, Tomaso ^{[2
]}

Zane, Lorenzo ^{[1
]}

机构：

[1] Univ Padua, Dept Biol, I-35121 Padua, Italy

[2] Univ Padua, Dept Publ Hlth Comparat Pathol & Vet Hygene, I-35020 Legnaro, Pd, Italy

来源：

CONSERVATION GENETICS RESOURCES | 2011年 / 3卷 / 02期

基金：

美国国家科学基金会;

关键词：

Pleuragramma antarcticum; Antarctic silverfish; Notothenioidei; Southern Ocean; Microsatellite; FIASCO protocol; EARLY-LIFE STAGES; DNA MARKERS; ROSS SEA; ALLELES;

D O I：

10.1007/s12686-010-9336-9

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We characterized nine polymorphic microsatellites by an enriched library from the Antarctic silverfish Pleuragramma antarcticum, a key species in the high Antarctic zone of the Southern Ocean. The number of alleles scored ranged from 7 to 39, whereas the observed and expected heterozygosity ranged from 0.4000 to 0.9750 and from 0.3943 to 0.9782, respectively. Cross-amplification was tested in the 3 species of the genus Chionodraco (Channichthyidae). These new microsatellites could potentially be useful in further investigations on P. antarcticum for which many questions on population structure and exposition to global environment change are still open to debate.

引用

页码：259 / 262

页数：4

共 13 条

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