Protein tyrosine dephosphorylation during copper-induced cell death in rice roots

被引:31
|
作者
Hung, Wan-Chi [1 ]
Huang, Dinq-Ding [1 ]
Chien, Pei-Shan [1 ]
Yeh, Chuan-Ming [1 ]
Chen, Po-Yu [1 ]
Chi, Wen-Chang [1 ]
Huang, Hao-Jen [1 ]
机构
[1] Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan
关键词
programmed cell death; signalling; tyrosine phosphorylation;
D O I
10.1016/j.chemosphere.2007.04.073
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Early signalling events that control the process of heavy metal-induced cell death are largely unknown in plants. In mammals protein tyrosine phosphorylation plays an important role in the activation of programmed cell death. We thus examined the involvement of tyrosine phosphorylation in Cu-induced rice cell death. This investigation demonstrates that Cu induces cell death and DNA fragmentation in rice root cells. In the presence of Cu, the level of phosphotyrosine accumulation declined in the band of 45 kDa, p45. To analyze the role of tyrosine dephosphorylation for the regulation of Cu-induced cell death more precisely, we increased levels of tyrosine phosphorylation using the protein tyrosine phosphatase inhibitor, sodium orthovanadate (Na3VO4). Treatment of rice roots with Na3VO4 blocked Cu-induced cell death and protein tyrosine dephosphorylation. In addition, the antioxidant GSH and the calcium chelator EGTA significantly abolished Cu-induced cell death and protein tyrosine dephosphorylation. These results provide evidence that dephosphorylation of a tyrosine-phosphorylated protein, p45, is an important step in the Cu-triggered signalling transduction pathway. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:55 / 62
页数:8
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