An efficient method for the rapid establishment of Epstein-Barr virus immortalization of human B lymphocytes

被引：49
作者：
Oh, HM
Oh, JM
Choi, SC
Kim, SW
Han, WC
Kim, TH
Park, DS
Jun, CD [1 ]
机构：
[1] Wonkwang Univ Sch Med, Dept Microbiol & Immunol, Iksan 570749, Chonbuk, South Korea
[2] Wonkwang Univ Sch Med, Digest Dis Res Inst, Iksan 570749, Chonbuk, South Korea
[3] Wonkwang Univ Sch Med, Genome Res Ctr Immune Disorders, Iksan 570749, Chonbuk, South Korea
来源：
CELL PROLIFERATION | 2003年 / 36卷 / 04期
关键词：
D O I：
10.1046/j.1365-2184.2003.00276.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Several methods have been developed for the immortalization of B lymphocytes by Epstein-Barr virus (EBV). We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester-induced EBV stock significantly improved immortalization efficiency and reduced the time between initiation and immortalization and cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in immune and autistic disorders.
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收藏
页码：191 / 197
页数：7
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