Vanillylacetone attenuates NLRP3 inflammasome mediated immune responses in murine bone marrow derived macrophages via NLRP3 alkylation

被引：7
作者：
Kim, Han-Bi [1 ]
Kwon, Sang-Chul [2 ]
Sun, Xiao [1 ]
Akther, Mahbuba [1 ]
Han, Jun-Hyuk [1 ]
Kim, Tae-Yeon [1 ]
Kang, Tae-Bong [3 ]
Lee, Kwang-Ho [1 ,3 ]
机构：
[1] Konkuk Univ, Grad Sch, Dept Appl Life Sci, BK21 Plus Glocal Educ Program Neutraceut Dev, Chungju, South Korea
[2] Korea Natl Univ Transportat, Coll Hlth & Life Sci, Dept Food Sci & Technol, Jeungpyeong Gun, South Korea
[3] Konkuk Univ, Coll Biomed & Hlth Sci, Res Inst Inflammatory Dis, Dept Biotechnol, Chungju, South Korea
来源：
JOURNAL OF FUNCTIONAL FOODS | 2020年 / 64卷
关键词：
Vanillylacetone; NLRP3; Inflammasome; Alkylation; Gout arthritis; ASC; PARTHENOLIDE; ACTIVATION; CASPASE-1; ZINGERONE; PROTEIN;
D O I：
10.1016/j.jff.2019.103655
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Vanillylacetone (VA) is widely used as a food additive owing to its sweet flavour. In this study, we have reported for the first time that VA has an inhibitory role in inflammasome activation. We examined the effect of VA on inflammasome activation and investigated the underlying physiological mechanisms. We verified that VA suppresses IL-1 beta and caspase-1 secretion, induced by NLRP3 inflammasome activation, in lipopolysaccharide (LPS)primed bone marrow-derived macrophages. ASC speck formation and oligomerization were also attenuated by VA treatment. VA potentially inhibits NLRP3 inflammasome activation via NLRP3 alkylation. The in vivo efficacy of VA on NLRP3 inflammasome activation was examined in an MSU-induced murine peritonitis model. IL-1 beta and neutrophil recruitment in peritoneal lavage fluid was significantly reduced by VA treatment, thus suggesting that VA could potentially be used as a therapeutic agent against NLRP3 inflammasome-associated diseases.
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页数：8
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