Half-embryo cocultivation technique for estimating the susceptibility of pea (Pisum sativum L.) and lentil (Lens culinaris Medik.) cultivars to Agrobacterium tumefaciens

被引：9
作者：
Lurquin, PF
Cai, ZL
Stiff, CM
Fuerst, EP
机构：
[1] Washington State Univ, Dept Genet & Cell Biol, Pullman, WA 99164 USA
[2] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA
来源：
MOLECULAR BIOTECHNOLOGY | 1998年 / 9卷 / 02期
基金：
美国国家科学基金会;
关键词：
Agrobacterium tumefaciens; gene transfer; lentils; peas; susceptibility;
D O I：
10.1007/BF02760819
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Longitudinally sliced embryonic axes from pea and lentil mature seeds cocultivated with A. tumefaciens carrying a gus reporter gene in its T-DNA provided a convenient means to evaluate the efficiency of gene transfer to tissues in different cultivars and cocultivation conditions. Use of this technique demonstrated wide variation in susceptibility to Agrobacterium among several pea and lentil commercial genotypes.
引用
收藏
页码：175 / 179
页数：5
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