Inhibitory Effect of MiR-449b on Cancer Cell Growth and Invasion through LGR4 in Non-Small-Cell Lung Carcinoma

被引：22

作者：

Yang, Dong ^{[1
]}

Li, Jin-song ^{[1
]}

Xu, Qian-yu ^{[1
]}

Xia, Tian ^{[1
]}

Xia, Jia-hong ^{[2
]}

机构：

[1] Huazhong Univ Sci & Technol, Union Hosp, Tong Med Coll, Dept Thorac Surg, Wuhan 430022, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Union Hosp, Tong Med Coll, Dept Cariovasc Surg, Wuhan 430022, Hubei, Peoples R China

来源：

CURRENT MEDICAL SCIENCE | 2018年 / 38卷 / 04期

关键词：

non-small-cell lung carcinoma; microRNA-449b; leucine rich repeat containing G protein-coupled receptor 4; growth; invasion; THYROID-CARCINOMA; TUMOR-SUPPRESSOR; DOWN-REGULATION; PROLIFERATION; MICRORNAS; METASTASIS; MIGRATION; GPR48;

D O I：

10.1007/s11596-018-1917-y

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Non-small-cell lung carcinoma (NSCLC) is one of the most frequently diagnosed malignancies worldwide. Previous studies have shown that microRNA-449b (miR-449b) functions as a tumor suppressor in many cancers. However, the role of miR-449b in NSCLC is still unknown. In the present study, miR-449b was significantly downregulated in NSCLC samples and cell lines. Bioinformatics analysis revealed that 3'-UTR region of leucine rich repeat containing G protein-coupled receptor 4 (LGR4) mRNA had putative complementary sequences to miR-449b,which was further confirmed by the luciferase assay. Western blotting showed that restoration of miR-449b in NSCLC cells decreased the expression of LGR4. Interestingly, over-expression of miR-449b inhibited growth and invasion of NSCLC cells in vitro. Furthermore, ectopic expression of LGR4 reversed miR-449b-suppressed proliferation and invasion of NSCLC cells. Therefore, the data of the present study demonstrate that miR-449b inhibits tumor cell growth and invasion by targeting LGR4 in NSCLC.

引用

页码：582 / 589

页数：8

共 30 条

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