Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti

被引:15
作者
Herbert, James D. K. [1 ]
Godfrey, Stephanie S. [1 ]
Bull, C. Michael [1 ]
Menz, R. Ian [1 ]
机构
[1] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia
基金
澳大利亚研究理事会;
关键词
Apicomplexa; Tuatara; Hepatozoon tuatarae; 18S rRNA; Ixodidae; Life cycle; HEMOGREGARINE; APICOMPLEXA; ISLAND;
D O I
10.1016/j.ijpara.2010.03.018
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The developmental stages of Hepatozoon tuatarae were elucidated in both the tuatara host, Sphenodon punctatus and the tick. Amblyomma sphenodonn PCR amplicons from A sphenodonn samples identified DNA matching H. tuatarae Dissection of tick samples showed oogenesis and sporogony occurring in the haemocoel of A sphenodonti with the average mature oocyst size being 236 x 228 pm. Partial sequence data of the parasite's small subunit ribosomal gene, obtained by PCR, was used for phylogenetic comparison. Characterisation of the H tuatarae lifecycle will help in conservation management of the tuatara. (C) 2010 Australian Society for Parasitology Inc Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1311 / 1315
页数:5
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