Aldehyde dismutase activity of yeast alcohol dehydrogenase

被引：14

作者：

Trivic, S

Leskova, V

Winston, GW

机构：

[1] Univ Novi Sad, Fac Technol, YU-21000 Novi Sad, Yugoslavia

[2] Fac Sci Novi Sad, YU-21000 Novi Sad, Yugoslavia

[3] N Carolina State Univ, Raleigh, NC 27695 USA

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 03期

关键词：

alcohol dehydrogenase; aldehyde dismutase; enzyme kinetics; Saccharomyces cerevisiae; yeast;

D O I：

10.1023/A:1005476115349

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Yeast alcohol dehydrogenase (EC 1.1.1.1) is able to catalyze the oxidation of acetaldehyde by NAD(+) with a concomitant formation of ethanol, at pH 8.8 and pH 7.1; the stoichiometry of aldehyde oxidation vs, ethanol formation is 2:1. This enzymatic reaction obeys the Michaelis-Menten kinetics and was characterized by a high K-M for acetaldehyde (68 mM) and a low k(cat) (2.3 s(-1)), at pH 8.8, 22 degrees C. There is no visible burst of NADH during the reaction, from pH 7.1-10.1. Therefore, we have concluded that the enzyme catalyzes an apparent dismutation of two molecules of acetaldehyde into a molecule of acetic acid and a molecule of ethanol.

引用

页码：231 / 234

页数：4

共 21 条

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