GENETIC ANALYSIS OF ESCHERICHIA COLI STRAINS CARRYING ENTEROPATHOGENIC ESCHERICHIA COLI (EPEC) MARKERS, ISOLATED FROM CHILDREN IN RIO DE JANEIRO CITY, BRAZIL

被引：2

作者：

Regua-Mangia, Adriana Hamond ^{[1
]}

Tardelli Gomes, Tania Aparecida ^{[2
]}

Costa Andrade, Joao Ramos ^{[3
]}

Midolli Vieira, Monica Aparecida ^{[2
]}

Martins Gonzalez, Alice Goncalves ^{[4
]}

Zahner, Viviane

Irino, Kinue ^{[5
]}

Teixeira, Lucia Martins ^{[6
]}

机构：

[1] Fundacao Oswaldo Cruz, Escola Nacl Saude Publ, Dept Ciencias Biol, BR-21041210 Rio De Janeiro, Brazil

[2] Univ Fed Sao Paulo, Sao Paulo, Brazil

[3] Univ Estado Rio De Janeiro, Rio De Janeiro, Brazil

[4] Univ Fed Fluminense, Rio De Janeiro, Brazil

[5] Inst Adolfo Lutz Registro, Sao Paulo, Brazil

[6] Univ Fed Rio de Janeiro, Rio De Janeiro, Brazil

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2003年 / 34卷

关键词：

enteropathogenic Escherichia coli; genetic diversity; virulence markers;

D O I：

10.1590/S1517-83822003000500013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the present study, 47 enteropathogenic Escherichia coli strains identified according to serotyping, presence of eae, bfp and EAF sequences, adherence phenotype and ability to induce attaching-effacing lesions were analyzed by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MLEE), and the presence of LEE genes (eae, espA, espB, tir) as well as the respective alleles. Amplification of LEE genes subtypes revealed 18 different pathotypes. Typing of the eae gene showed that most strains contained nontypable intimin (42%) followed by beta(35%), gamma and alpha genes (12% each). PFGE analysis revealed a variable degree of polymorphism among isolates and, in general, no clear correlation was observed among PFGE profiles and the virulence markers identified. Otherwise, grouping based oil M LEE analysis showed a close association between eae allele and clonal cluster distribution leading us to indicate the eae profile as a promising marker to establish relatedness among such microorganisms.

引用

页码：38 / 41

页数：4

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