The effect of top-layer chemistry on the formation of supported lipid bilayers on polyelectrolyte multilayers: primary versus quaternary amines

被引：9

作者：

Diamanti, E. ^{[1
]}

Andreozzi, P. ^{[1
]}

Anguiano, R. ^{[2
]}

Yate, L. ^{[1
]}

Gregurec, D. ^{[1
]}

Politakos, N. ^{[1
]}

Ziolo, R. F. ^{[2
]}

Donath, E. ^{[3
]}

Moya, S. E. ^{[1
]}

机构：

[1] CIC BiomaGUNE, Soft Matter Nanotechnol Grp, Paseo Miramon 182 C, San Sebastian 20009, Guipuzcoa, Spain

[2] Ctr Invest Quim Aplicada, Dept Mat Avanzados, Blvd Enrique Reyna Hermosillo 140, Saltillo 25250, Coahuila, Mexico

[3] Univ Leipzig, Fac Med, Inst Biophys & Med Phys, D-04107 Leipzig, Germany

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2016年 / 18卷 / 47期

关键词：

QUARTZ-CRYSTAL MICROBALANCE; VESICLE ADSORPTION; PHOSPHOLIPID-BILAYERS; PLANAR BILAYERS; MEMBRANES; SURFACE; DIFFUSION; RECOVERY; MICROCAPSULES; TEMPERATURE;

D O I：

10.1039/c6cp06258j

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The influence of the surface chemistry of polyelectrolyte multilayers (PEMs) on the formation of lipid bilayers is studied here for PEMs with either polyallylamine hydrochloride (PAH) or polydiallyldimethyl-ammonium chloride (PDADMAC) as a polycation as a top layer, and polystyrene sulfonate (PSS) as a polyanion. Small unilamellar vesicles (SUVs) composed of phosphatidyl choline and phosphatidyl serine at a 50 : 50 molar ratio are deposited on top of the PEM films. The assembly of the SUVs into bilayers is studied via a quartz crystal microbalance with dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP). SUV deposition on PDADMAC/PSS results in vesicle adsorption while on PAH/ PSS under the same conditions a bilayer is formed mainly due to weak interactions between the quaternary amines of PDADMAC. FRAP measurements confirm that SUVs are not fused on top of PDADMAC/PSS. The effect of phosphate ions, in solution, on the formation of lipid bilayers is also analysed. X-ray photoelectron spectroscopy shows the complexation of phosphate salts to the primary amines of PAH and no interaction with the quaternary amines of PDADMAC. zeta-potential measurements show a potential close to 0 for the PAH/PSS multilayers in PBS while PDADMAC/PSS displays a potential of 25 mV. A model is presented for the formation of lipid bilayers on PAH/PSS PEMs taking into account the role of phosphate ions in decreasing the electrostatic interactions between SUVs and PEMs and the formation of hydrogen bonds between the phospholipids and the primary amines of PAH.

引用

页码：32396 / 32405

页数：10

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