Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis

被引：3

作者：

Marks, Guido ^{[1
]}

Fagundes, Djalma Jose ^{[2
]}

Ynouye, Celso Massaschi ^{[3
]}

Jardim Cury Pontes, Elenir Rose ^{[4
]}

Takita, Luiz Carlos ^{[3
]}

Siufi do Amaral, Eva Gloria ^{[3
]}

Teruya, Roberto ^{[3
]}

Paes, Manoel Catarino ^{[3
]}

Brasileiro, Jose Lacerda ^{[3
]}

Aydos, Ricardo Dutra ^{[3
]}

机构：

[1] Univ Fed Sao Paulo, Dept Surg, Postgraduat Program Surg & Experimentat, Sao Paulo, Brazil

[2] Univ Fed Sao Paulo, Dept Surg, Div Operat Techn & Expt Surg, Sao Paulo, Brazil

[3] UFMS MS, Dept Surg, Sao Luis, Maranhao, Brazil

[4] UFMS MS, Dept Food Technol & Publ Hlth, Sao Luis, Maranhao, Brazil

来源：

ACTA CIRURGICA BRASILEIRA | 2008年 / 23卷 / 02期

关键词：

apoptosis; azoxymethane; cancer; colon; inositol 1,4,5 trisphosphate receptors; computer-assisted image processing; phytic acid; rats;

D O I：

10.1590/S0102-86502008000200008

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Purpose: To study the effect of the modulation of inositol hexaphosphate (IP6) in the biological immunohistochemistry expression of cellular signaling marker apoptosis, in model of carcinogenesis of colon induced by azoxymethane (AOM). Methods: Wistar rats (N= 112) distributed in 4 groups (n=28): Control; B, AOM (5 mg kg(-1), 2x, to break week 3); C, IP6 (in water 1 %, six weeks); D, IP6+AOM. Weekly euthanasia (n=7), from week three. Immunohistochemistry of ascendant colon with biological marker inositol 1,4,5 triphosphate receptor type III (Itpr3). Quantification of the immune-expression with use of computer-assisted image processing. Analysis statistics of the means between groups, weeks in groups, groups in weeks, and established significance when p <= 0.05. Results: One proved significant difference between groups in the expression of Itpr3, p < 0.0001;with Itpr3 reduction of BxD group, p < 0.001. Conclusion: Inositol hexaphosphate promotes modulation of biological markers with reduction of Itpr3 in carcinogenesis of colon.

引用

页码：157 / 164

页数：8

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