GacA directly regulates expression of several virulence genes in Pseudomonas syringae pv. tabaci 11528

被引：23
作者：
Cha, Ji Young [1 ]
Lee, Dong Gwang [1 ]
Lee, Jun Seung [1 ]
Oh, Jeong-Il [1 ]
Baik, Hyung Suk [1 ]
机构：
[1] Pusan Natl Univ, Coll Nat Sci, Dept Microbiol, Pusan 609735, South Korea
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 417卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
GacA; Iron availability; Quantitative RT-PCR; Electrophoretic mobility shift assay; Quorum sensing; 2-COMPONENT SYSTEM; RESPONSE REGULATOR; SUPPRESSION; MODULATE; IRON; FUR; HRP;
D O I：
10.1016/j.bbrc.2011.11.124
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A two-component system comprising GacS and GacA affects a large number of traits in many Gram-negative bacteria. However, the signals to which GacS responds, the regulation mechanism for GacA expression, and the genes GacA controls are not yet clear. In this study, several phenotypic tests and tobacco-leaf pathogenicity assays were conducted using a gacA deletion mutant strain (BL473) of Pseudomonas syringae pv. tabaci 11528. To determine the regulation mechanism for gacA gene expression and to identify GacA-regulated genes, we conducted quantitative RT-PCR and electrophoretic mobility shift assay (EMSA) experiments. The results indicated that virulence traits related to the pathogenesis of P. syringae pv. tabaci 11528 are regulated coordinately by GacA and iron availability. They also revealed that several systems coordinately regulate gacA gene expression in response to iron concentration and bacterial cell density and that GacA and iron together control the expression of several virulence genes. EMSA results provided genetic and molecular evidence for direct control of virulence genes by GacA. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：665 / 672
页数：8
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