[11C]Acetate rest-stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats

被引:27
作者
Croteau, Etienne [2 ,3 ]
Gascon, Suzanne [2 ,3 ]
Bentourkia, M'hamed [2 ,3 ]
Langlois, Rejean [2 ,3 ]
Rousseau, Jacques A. [2 ,3 ]
Lecomte, Roger [2 ,3 ]
Benard, Francois [1 ]
机构
[1] Univ British Columbia, Dept Radiol, Div Nucl Med, Vancouver, BC, Canada
[2] Univ Sherbrooke, Dept Med Nucl & Radiobiol, Sherbrooke, PQ J1K 2R1, Canada
[3] CHUS, Sherbrooke Mol Imaging Ctr, Etienne LeBel Clin Res Ctr, Sherbrooke, PQ, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
C-11]Acetate; Cardiovascular reserve; Myocardial perfusion; Myocardial oxygen consumption; Rest-stress study; Small-animal PET imaging; Dobutamine; Congestive heart failure; POSITRON-EMISSION-TOMOGRAPHY; C-11; ACETATE; BLOOD-FLOW; OXIDATIVE-METABOLISM; PERFORMANCE EVALUATION; IN-VIVO; PET; EFFICIENCY; CARBON-11-ACETATE; CARDIOMYOPATHY;
D O I
10.1016/j.nucmedbio.2011.07.010
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
This study describes an [C-11]acetate rest-stress method to obtain an indirect estimate of myocardial blood flow (MBF) and myocardial oxygen consumption (MVO2) in rats. Doxorubicin cardiotoxicity was used to test the usefulness of this approach for the assessment of congestive heart failure. Methods: [C-11]Acetate rest stress studies have been used in clinical research to assess the capacity of the coronary arteries to respond to stress. In this article, we used this approach to assess the cardiotoxicity of doxorubicin in a rat model. The method was first validated in a group of healthy rats and then used to follow the effect of doxorubicin chemotherapy on cardiac function. The effect of doxorubicin on myocardial perfusion and oxygen consumption reserve was measured at rest and under dobutamine stimulation. Results: Validation of the protocol showed a good correlation between the MBF and MVO2 (r(2)=.68). The doxorubicin-treated group showed a significant (P=.04) decrease in cardiovascular perfusion reserve at 1.3 +/- 0.2 compared with the control animals at 1.6 +/- 0.2. Similar results were obtained for the MVO2 reserve (treated 1.8 +/- 0.4 vs. controls 2.3 +/- 0.3; P=.02). Conclusions: We describe an [C-11]acetate PET rest stress protocol for the assessment of congestive heart failure in rats and its application to the follow-up of cardiotoxicity under doxorubicin chemotherapy. This is a rapid and reliable approach to the measurement of cardiac perfusion and oxygen consumption reserve that could be applied to the development of new strategies to reduce the cardiotoxicity of anthracycline. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:287 / 294
页数:8
相关论文
共 39 条
[1]  
ABLAD B, 1975, ACTA PHARMACOL TOX, V36, P7
[2]   Non-invasive estimation of myocardial efficiency using positron emission tomography and carbon-11 acetate - comparison between the normal and failing human heart [J].
Bengel, FM ;
Permanetter, B ;
Ungerer, M ;
Nekolla, S ;
Schwaiger, M .
EUROPEAN JOURNAL OF NUCLEAR MEDICINE, 2000, 27 (03) :319-326
[3]   Cardiac studies in rats with 11C-acetate and PET:: A comparison with 13N-ammonia [J].
Bentourkia, M ;
Croteau, É ;
Langlois, R ;
Aliaga, A ;
Cadorette, J ;
Bénard, F ;
Lesur, O ;
Lecomte, R .
IEEE TRANSACTIONS ON NUCLEAR SCIENCE, 2002, 49 (05) :2322-2327
[4]   Performance Evaluation of the LabPET12, a Large Axial FOV APD-based Digital PET Scanner [J].
Bergeron, M. ;
Cadorette, J. ;
Bureau-Oxton, C. ;
Beaudoin, J. -F. ;
Tetrault, M. -A. ;
Leroux, J. -D. ;
Lepage, M. D. ;
Robert, G. ;
Fontaine, R. ;
Lecomte, R. .
2009 IEEE NUCLEAR SCIENCE SYMPOSIUM CONFERENCE RECORD, VOLS 1-5, 2009, :4017-+
[5]   Performance Evaluation of the LabPET APD-Based Digital PET Scanner [J].
Bergeron, Melanie ;
Cadorette, Jules ;
Beaudoin, Jean-Francois ;
Lepage, Martin D. ;
Robert, Ghislain ;
Selivanov, Vitali ;
Tetrault, Marc-Andre ;
Viscogliosi, Nicolas ;
Norenberg, Jeffrey P. ;
Fontaine, Rejean ;
Lecomte, Roger .
IEEE TRANSACTIONS ON NUCLEAR SCIENCE, 2009, 56 (01) :10-16
[6]   Coronary collateral perfusion in patients with coronary artery disease: effect of metoprolol [J].
Billinger, M ;
Raeber, L ;
Seiler, C ;
Windecker, S ;
Meier, B ;
Hess, OM .
EUROPEAN HEART JOURNAL, 2004, 25 (07) :565-570
[7]  
BROWN MA, 1989, J NUCL MED, V30, P187
[8]   NONINVASIVE ASSESSMENT OF CANINE MYOCARDIAL OXIDATIVE-METABOLISM WITH C-11 ACETATE AND POSITRON EMISSION TOMOGRAPHY [J].
BROWN, MA ;
MYEARS, DW ;
BERGMANN, SR .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1988, 12 (04) :1054-1063
[9]  
BUCK A, 1991, J NUCL MED, V32, P1950
[10]   Myocardial perfusion during long-term angiotensin-converting enzyme inhibition or β-blockade in patients with essential hypertension [J].
Buus, NH ;
Bottcher, M ;
Jorgensen, CG ;
Christensen, KL ;
Thygesen, K ;
Nielsen, TT ;
Mulvany, MJ .
HYPERTENSION, 2004, 44 (04) :465-470