Phosphorylation of calmodulin by permeabilized fibroblasts overexpressing the human epidermal growth factor receptor

被引:12
作者
DeFrutos, T [1 ]
MartinNieto, J [1 ]
Villalobo, A [1 ]
机构
[1] CSIC,INST INVEST BIOMED,E-28029 MADRID,SPAIN
关键词
cell permeabilization; phosphocalmodulin; tyrosine kinase;
D O I
10.1515/bchm.1997.378.1.31
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Detergent-permeabilized EGFR-T17 fibroblasts, which overexpress the human epidermal growth factor (EGF) receptor, phosphorylate both poly-L-(glutamic acid, tyrosine) and exogenous calmodulin in an EGF-stimulated manner. Phosphorylation of calmodulin requires the presence of cationic polypeptides, such as poly-L-(lysine) or histones, which exert a biphasic effect toward calmodulin phosphorylation. Optimum cationic polypeptide/calmodulin molar ratios of 0.3 and 7 were determined for poly-L-(lysine) and histones, respectively. Maximum levels of calmodulin phosphorylation were attained in the absence of free calcium, and a strong inhibition of this process was observed at very low concentrations (Ki= 0.2 mu M) of this cation. The incorporation of phosphate into calmodulin occurred predominantly on tyrosine residue(s) and was stimulated 34-fold by EGF.
引用
收藏
页码:31 / 37
页数:7
相关论文
共 45 条
[1]   CALMODULIN AND CALMODULIN-BINDING PROTEINS IN THE NUCLEUS [J].
BACHS, O ;
AGELL, N ;
CARAFOLI, E .
CELL CALCIUM, 1994, 16 (04) :289-296
[2]   CALCIUM AND CALMODULIN FUNCTION IN THE CELL-NUCLEUS [J].
BACHS, O ;
AGELL, N ;
CARAFOLI, E .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1113 (02) :259-270
[3]   PHOSPHORYLATION OF CALMODULIN BY THE EPIDERMAL-GROWTH-FACTOR-RECEPTOR TYROSINE KINASE [J].
BENGURIA, A ;
HERNANDEZPERERA, O ;
MARTINEZPASTOR, MT ;
SACKS, DB ;
VILLALOBO, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 224 (03) :909-916
[4]   Regulatory interaction between calmodulin and the epidermal growth factor receptor [J].
Benguria, A ;
MartinNieto, J ;
Benaim, G ;
Villalobo, A .
RECEPTOR ACTIVATION BY ANTIGENS, CYTOKINES, HORMONES, AND GROWTH FACTORS, 1995, 766 :472-476
[5]   PHOSPHORYLATION OF CALMODULIN BY PLASMA-MEMBRANE-ASSOCIATED PROTEIN KINASE(S) [J].
BENGURIA, A ;
SORIANO, M ;
JOYAL, JL ;
SACKS, DB ;
VILLALOBO, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 234 (01) :50-58
[6]  
BENGURIA A, 1993, BIOREGULADORES, V2, P74
[7]  
BIENER Y, 1991, J BIOL CHEM, V266, P17369
[8]   INTERACTION OF CALCIUM AND CALMODULIN IN THE PRESENCE OF SODIUM DODECYL-SULFATE [J].
BURGESS, WH ;
JEMIOLO, DK ;
KRETSINGER, RH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 623 (02) :257-270
[9]   RECEPTORS FOR EPIDERMAL GROWTH-FACTOR AND OTHER POLYPEPTIDE MITOGENS [J].
CARPENTER, G .
ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :881-914
[10]  
COUNTAWAY JL, 1992, J BIOL CHEM, V267, P1129