Absorption of the green fluorescent protein chromophore anion in the gas phase studied by a combination of FTICR mass spectrometry with laser-induced photodissociation spectroscopy

被引：32

作者：

Chingin, Konstantin ^{[1
]}

Balabin, Roman M. ^{[1
]}

Frankevich, Vladimir ^{[1
]}

Barylyuk, Konstantin ^{[1
]}

Nieckarz, Robert ^{[1
]}

Sagulenko, Pavel ^{[1
]}

Zenobi, Renato ^{[1
]}

机构：

[1] ETH, Dept Chem & Appl Biosci, Zurich, Switzerland

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2011年 / 306卷 / 2-3期

关键词：

GFP chromophore; HBDI anion; Gas-phase absorption; Action spectroscopy; FTICR-MS; STATE; SOLVATOCHROMISM; CASPT2//CASSCF; ISOMERIZATION; PHOTOPHYSICS;

D O I：

10.1016/j.ijms.2011.01.014

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

The optical absorption of the green fluorescent protein chromophore anion (HBDI-) in the gas phase has been addressed by a number of experimental and theoretical studies; however, there is no consensus on its spectral characteristics yet. In this report, the intrinsic absorption of HBDI- was probed by photo-induced dissociation ("action") spectroscopy using discrete lines of a continuous-wave (CW) laser source. The observed spectral profile of dissociation efficiency revealed a pronounced dependence on the laser irradiance. Dissociation at high irradiance is governed by multiple-photon absorption processes whose efficiency peaks between 476 and 488 nm. At very low irradiance (<0.4 mW cm(-2)) dissociation of gas-phase HBDI- is mostly promoted by single-photon transitions and suggests an intrinsic absorption maximum of gas-phase HBDI- <476 nm, blue-shifted compared to earlier results obtained with pulsed lasers. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：241 / 245

页数：5

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