Portable GMR Handheld Platform for the Detection of Influenza A Virus

被引：103
作者：
Wu, Kai [1 ]
Klein, Todd [1 ]
Krishna, Venkatramana D. [2 ]
Su, Diqing [1 ,3 ]
Perez, Andres M. [2 ]
Wang, Jian-Ping [1 ]
机构：
[1] Univ Minnesota, Dept Elect & Comp Engn, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA
[3] Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA
来源：
ACS SENSORS | 2017年 / 2卷 / 11期
基金：
美国国家科学基金会;
关键词：
Influenza A virus; point-of-care device; giant magnetoresistive; quantitative immunoassay platform; nonclinical; CARBON NANOTUBES; MAGNETIC NANOPARTICLES; PROTEIN; SENSOR; SYSTEM; SWINE; H5N1; QUANTIFICATION; IMMUNOSENSOR; IMMUNOASSAY;
D O I：
10.1021/acssensors.7b00432
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas:We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.
引用
收藏
页码：1594 / 1601
页数：8
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