Novel electrochemical biosensor based on core-shell nanostructured composite of hollow carbon spheres and polyaniline for sensitively detecting malathion

被引：83

作者：

He, Linghao ^{[1
]}

Cui, Bingbing ^{[1
]}

Liu, Jiameng ^{[1
]}

Song, Yingpan ^{[1
]}

Wang, Minghua ^{[1
]}

Peng, Donglai ^{[1
]}

Zhang, Zhihong ^{[1
]}

机构：

[1] Zhengzhou Univ Light Ind, Henan Prov Key Lab Surface Interface Sci, Zhengzhou 450002, Henan, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 258卷

基金：

中国国家自然科学基金;

关键词：

Hollow carbon spheres; Polyaniline; Electrochemical biosensor; Acetylcholine esterase; Detection of malathion; DOPED SNO2 NANOPARTICLES; ACETYLCHOLINESTERASE BIOSENSOR; ORGANOPHOSPHORUS PESTICIDES; GLUCOSE BIOSENSOR; HIGH-PERFORMANCE; MODIFIED ELECTRODE; OXYGEN-REDUCTION; SENSOR; OXIDE; NANOCOMPOSITE;

D O I：

10.1016/j.snb.2017.11.161

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel electrochemical biosensor was constructed by anchoring acetylcholine esterase (AChE) onto the core-shell nanostructured composite of hollow carbon spheres coated with needle-like polyaniline(denoted by HCS@PANI), and used for rapid determination of malathion, which was established based on the chemisorption/desorption process of AChE used as an indicator. The uniformly distributed HCS was wrapped with the needle-like PANI nanowires to form the HCS@PANI nanocomposite, which not only exhibits high specific surface area and chemical functionality but also possesses strong electrochemical activity. After AChE was adsorbed onto the HCS@PANI nanocomposite, the electrochemical catalysis of AChE/HCS@PANI-based biosensor towards acetylthiocholine chloride (ATCl) was inhibited in the presence of malathion, which was applied to detect malathion, and the biosensor exhibited a low detection limit of 0.16 ng mL(-1) within a linear range from 1.0 ng mL(-1) to 10 mu g mL(-1) of the malathion concentration. Also, the results suggested that the developed electrochemical biosensor showed high selectivity, good reproducibility, and applicability for malathion detection, indicating that the proposed method using the HCS@PANI nanocomposite as the sensitive layer for the AChE immobilization and catalysis of ATCl has potential application in high-sensitive pesticide determinations. (c) 2017 Elsevier B.V. All rights reserved.

引用

页码：813 / 821

页数：9

共 69 条

[1] A novel glucose sensor using lutetium phthalocyanine as redox mediator in reduced graphene oxide conducting polymer multifunctional hydrogel [J].