Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations

被引：241

作者：

Li, Yang ^{[1
]}

Liu, Lina ^{[2
]}

Wan, Peng ^{[3
]}

Zhai, Zanjing ^{[1
]}

Mao, Zhenyang ^{[1
]}

Ouyang, Zhengxiao ^{[1
]}

Yu, Degang ^{[1
]}

Sun, Qi ^{[4
]}

Tan, Lili ^{[3
]}

Ren, Ling ^{[3
]}

Zhu, Zhenan ^{[1
]}

Hao, Yongqiang ^{[1
]}

Qu, Xinhua ^{[1
]}

Yang, Ke ^{[3
]}

Dai, Kerong ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China

[3] Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Radiol, Shanghai, Peoples R China

来源：

BIOMATERIALS | 2016年 / 106卷

基金：

中国国家自然科学基金; 上海市自然科学基金;

关键词：

Osteomyelitis; Antibacterial activity; Biodegradable; Mg-Cu alloy; STAPHYLOCOCCUS-AUREUS; PSEUDOMONAS-AERUGINOSA; CORROSION-RESISTANCE; BIOFILM FORMATION; STAINLESS-STEEL; CHARGE-TRANSFER; RABBIT MODEL; LOADED PMMA; COPPER; TITANIUM;

D O I：

10.1016/j.biomaterials.2016.08.031

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Treatment of chronic osteomyelitis (bone infection) remains a clinical challenge; in particular, it requires an implantable material with improved antibacterial activity. Here, we prepared biodegradable magnesium (Mg)-copper (Cu) alloys with different Cu contents (0.05, 0.1, and 0.25 wt%) and assessed their potential for treating methicillin-resistant Staphylococcus aureus-induced osteomyelitis. We evaluated the microstructures, mechanical properties, corrosion behavior, and ion release of the alloys in vitro, and their biocompatibility and antibacterial activity in vitro and in vivo. The antibacterial activity of the Mg Cu alloys in vitro was demonstrated by microbiological counting assays, bacterial viability assays, biofilm formation observations, and the expression of biofilm, virulence, and antibiotic-resistance associated genes. The antibacterial activity of Mg-Cu alloys in vivo was confirmed by imaging examination, microbiological cultures, and histopathology. The biocompatibility of Mg-Cu alloys was confirmed by cell proliferation, vitality, and morphology assays in vitro and Cu2+ or Mg2+ ion assays, blood biochemical tests, and histological evaluation in vivo. The alloy containing 0.25 wt% Cu exhibited the highest antibacterial activity among the tested alloys, with favorable biocompatibility. Collectively, our results indicate the potential utility of Mg -Cu alloy implants with 0.25 wt% Cu in treating orthopedic infections. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：250 / 263

页数：14

共 75 条

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