Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division

被引:213
作者
Yingling, Jessica [1 ,2 ,4 ]
Youn, Yong Ha [1 ,4 ]
Darling, Dawn [1 ,4 ]
Toyo-Oka, Kazuhito [3 ,4 ]
Pramparo, Tiziano [1 ,4 ]
Hirotsune, Shinji [3 ]
Wynshaw-Boris, Anthony [1 ,4 ]
机构
[1] UCSD Sch Med, Dept Med, Ctr Human Genet & Genom, La Jolla, CA 92098 USA
[2] UCSD Sch Med, Biomed Sci Grad Program, La Jolla, CA 92098 USA
[3] Osaka City Univ, Grad Sch Med, Dept Genet Dis Res, Osaka 5458585, Japan
[4] UCSD Sch Med, Dept Pediat, Ctr Human Genet & Genom, La Jolla, CA 92098 USA
关键词
D O I
10.1016/j.cell.2008.01.026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mitotic spindle orientation and plane of cleavage in mammals is a determinant of whether division yields progenitor expansion and/or birth of new neurons during radial glial progenitor cell (RGPC) neurogenesis, but its role earlier in neuroepithelial stem cells is poorly understood. Here we report that Lis1 is essential for precise control of mitotic spindle orientation in both neuroepithelial stem cells and radial glial progenitor cells. Controlled gene deletion of Lis1 in vivo in neuroepithelial stem cells, where cleavage is uniformly vertical and symmetrical, provokes rapid apoptosis of those cells, while radial glial progenitors are less affected. Impaired cortical microtubule capture via loss of cortical dynein causes astral and cortical microtubules to be greatly reduced in Lis1-deficient cells. Increased expression of the LIS/dynein binding partner NDEL1 restores cortical microtubule and dynein localization in Lis1-deficient cells. Thus, control of symmetric division, essential for neuroepithelial stem cell proliferation, is mediated through spindle orientation determined via LIS1/NDEL1/dynein-mediated cortical microtubule capture.
引用
收藏
页码:474 / 486
页数:13
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