Mass Spectrometry-Based Approaches to Understand the Molecular Basis of Memory

被引:6
作者
Pontes, Arthur H. [1 ]
de Sousa, Marcelo V. [1 ]
机构
[1] Univ Brasilia, Dept Cell Biol, Lab Prot Chem & Biochem, Brasilia, DF, Brazil
来源
FRONTIERS IN CHEMISTRY | 2016年 / 4卷
关键词
memory; hippocampus; synapse; long-term potentiation (LTP); mass spectrometry; selected reaction monitoring (SRM); quantitative proteomics; LONG-TERM POTENTIATION; DEPENDENT SYNAPTIC PLASTICITY; COMPLEX PROTEIN MIXTURES; ELEMENT-BINDING PROTEIN; QUANTITATIVE PROTEOMICS; AMPA RECEPTORS; LATE-PHASE; AREA CA1; ABSOLUTE QUANTIFICATION; LASTING POTENTIATION;
D O I
10.3389/fchem.2016.00040
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The central nervous system is responsible for an array of cognitive functions such as memory, learning, language, and attention. These processes tend to take place in distinct brain regions; yet, they need to be integrated to give rise to adaptive or meaningful behavior. Since cognitive processes result from underlying cellular and molecular changes, genomics and transcriptomics assays have been applied to human and animal models to understand such events. Nevertheless, genes and RNAs are not the end products of most biological functions. In order to gain further insights toward the understanding of brain processes, the field of proteomics has been of increasing importance in the past years. Advancements in liquid chromatography-tandem mass spectrometry (LC-MS/MS) have enabled the identification and quantification of thousands of proteins with high accuracy and sensitivity, fostering a revolution in the neurosciences. Herein, we review the molecular bases of explicit memory in the hippocampus. We outline the principles of mass spectrometry (MS)-based proteomics, highlighting the use of this analytical tool to study memory formation. In addition, we discuss MS-based targeted approaches as the future of protein analysis.
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页数:12
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