Quantum dot ex vivo labeling of neuromuscular synapses

被引：18

作者：

Orndorff, Rebecca L. ^{[1
]}

Warnement, Michael R. ^{[1
]}

Mason, John N. ^{[2
]}

Blakely, Randy D. ^{[2
,4
,5
]}

Rosenthal, Sandra J. ^{[1
,2
,3
]}

机构：

[1] Vanderbilt Univ, Dept Chem, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Dept Pharmacol, Nashville, TN 37235 USA

[3] Vanderbilt Univ, Dept Phys, Nashville, TN 37235 USA

[4] Vanderbilt Univ, Vanderbilt Sch Med, Dept Psychiat, Nashville, TN 37235 USA

[5] Vanderbilt Univ, Vanderbilt Sch Med, Ctr Mol Neurosci, Nashville, TN 37235 USA

来源：

NANO LETTERS | 2008年 / 8卷 / 03期

关键词：

D O I：

10.1021/nl072460x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nicotinic receptors (nAchRs) are responsible for fast excitatory signaling by the neurotransmitter acetylcholine (Ach). They are present on the postsynaptic membrane at neuromuscular junctions (NMJs) and also at brain synapses. alpha-Bungarotoxin (alpha-BTX), a high-affinity nAchR antagonist, inhibits Ach binding and neurotransmission.(1,2) Here we demonstrate biotinylated alpha-BTX, bound to native mouse diaphragm nAchRs, can be quantified and visualized ex vivo using streptavidin-conjugated quantum dots. This approach provides a novel methodology for the direct assessment of the presence and mobility of neurotransmitter receptors in native tissue.

引用

页码：780 / 785

页数：6

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