Characterisation of a cyclophilin isoform in Trypanosoma cruzi

被引：24

作者：

Búa, J

Åslund, L

Pereyra, N

García, GA

Bontempi, EJ

Ruiz, AM

机构：

[1] Inst Nacl Parasitol Dr M Fatala Chaben, ANLIS, RA-1063 Buenos Aires, DF, Argentina

[2] Dept Genet & Pathol, Rudbeck Lab, SE-75185 Uppsala, Sweden

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 200卷 / 01期

关键词：

cyclophilin; peptidyl prolyl cis-trans isomerase activity; cyclosporin A inhibition; Trypanosoma cruzi;

D O I：

10.1016/S0378-1097(01)00193-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The immunosuppressive drug cyclosporin A (CsA) has shown antiparasitic activity against several protozoans and helminths, when complexed to proteins called cyclophilins (CyPs). In this paper, the molecular characterisation of one member of the CyP family in Trypanosoma cruzi is reported. TcCyP19 gene proved to be highly conserved compared to CyPs from other organisms and was highly homologous to a Trypanosoma brucei brucei CyPA. This gene was expressed in Escherichia toll and the purified recombinant protein exhibited a peptidyl prolyl cis-trans isomerase activity that was inhibited by CsA (IC50 = 18.4 +/- 0.8 nM). The TcCyP19 gene was located on two chromosomal bands in T. cruzi CL Brener clone. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：43 / 47

页数：5

共 22 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] The antiparasite effects of cyclosporin A: Possible drug targets and clinical applications [J].