Hepatoprotective effects of total phenylethanoid glycosides from Acanthus ilicifolius L. against carbon tetrachloride-induced hepatotoxicity

被引：24
作者：
Zhang, Meng-Qi [1 ,2 ,3 ]
Ren, Xia [1 ,2 ]
Zhao, Qing [1 ,2 ]
Yue, Shi-Jun [1 ,2 ]
Fu, Xiu-Mei [1 ,4 ]
Li, Xin [1 ,2 ]
Chen, Kai-Xian [5 ,6 ]
Guo, Yue-Wei [5 ,6 ]
Shao, Chang-Lun [1 ,2 ]
Wang, Chang-Yun [1 ,2 ]
机构：
[1] Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Minist Educ China, Qingdao 266003, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
[3] Qilu Univ Technol, Engn Res Ctr Zebrafish Models Human Dis & Drug Sc, Biol Inst, Key Lab Biosensor Shandong Prov,Shandong Acad Sci, Jinan 250103, Peoples R China
[4] Ocean Univ China, Coll Econ, Qingdao 266100, Peoples R China
[5] Pilot Natl Lab Marine Sci & Technol, Open Studio Druggabil Res Marine Nat Prod, Qingdao 266237, Peoples R China
[6] Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
来源：
JOURNAL OF ETHNOPHARMACOLOGY | 2020年 / 256卷
基金：
中国国家自然科学基金;
关键词：
Acanthus ilicifolius L; Total phenylethanoid glycosides; Hepatoprotective; Antioxidant; ACUTE LIVER-INJURY; ANTIOXIDANT ACTIVITY; ACTEOSIDE; POLYSACCHARIDES; EXTRACT; DAMAGE;
D O I：
10.1016/j.jep.2020.112795
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Ethnopharmacological relevance: Acanthus ilicifolius L. has been used as a folk medicine in the treatment of acute and chronic hepatitis in China for a long time. Phenylethanoid glycosides are one of main components in A. ilicifolius L. Aim of the study: The aim of present study was to assess the hepatoprotective activities of total phenylethanoid glycosides from A. ilicifolius L. (APhGs) against carbon tetrachloride (CCl4)-induced liver injury in vivo and in vitro. Materials and method: The APhGs was separated by resin column chromatography. The purity of total phenylethanoid glycosides was determined by UV-Vis spectrophotometry using acteoside as a standard. The hepatoprotective activities of APhGs against CCl4-induced liver injury were performed on experimental mice and L-02 hepatocytes. Moreover, the antioxidant activities of APhGs were tested in vitro. Results: The results showed that pre-administration of APhGs to mice decreased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, and improved superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) level in serum and liver tissue induced by CCl4. Specifically, the SOD activities of APhGs-H and APhGs-M treatment groups were stronger than that of silymarin treatment group. The protective activities of APhGs were confirmed by histopathological results. Moreover, immunohistochemical analysis showed that APhGs could remarkably down-regulate the protein expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-beta (IL-beta). In vitro experiment, APhGs was observed to increase L-02 hepatocyte viability against CCl4-induced hepatotoxicity. In addition, antioxidation assays revealed that APhGs showed 2,2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and ferric reducing ability. Conclusion: Overall, total phenylethanoid glycosides from A. ilicifolius L. displayed promising hepatoprotective effects. These results offer a support for the medicine uses of A. ilicifolius L.
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页数：8
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