Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma

被引:2
作者
He, Jiaoxia [1 ]
Zheng, Zhongling [1 ]
Liu, Tingting [1 ]
Ao, Yupei [1 ]
Yang, Yixuan [1 ]
Hu, Huaidong [1 ]
机构
[1] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Key Lab Mol Biol Infect Dis,Minis, Chongqing 400000, Peoples R China
关键词
ALPHA-FETOPROTEIN; ALPHA-1-ACID GLYCOPROTEIN; SURVEILLANCE; COMBINATION; DIAGNOSIS; PROTEIN;
D O I
10.1038/s41598-022-18894-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Saliva is rich in proteins, DNA, RNA and microorganisms, and can be regarded as a biomarker library. In order to explore a noninvasive and simple means of early screening for liver cancer, proteomics was used to screen salivary markers of hepatitis B associated liver cancer. We used mass spectrometry coupled isobaric tags for relative and absolute quantitation (iTRAQ)-technology to identify differentially expressed proteins (DEPs). Western blot, immunohistochemistry and enzyme linked immunosorbent assay were used to detect marker expression of in tissues and saliva. Statistical analysis was used to analyze the diagnostic efficacy of the markers was analyzed through statistical analyses. By comparing the hepatocellular carcinoma (HCC) group with non-HCC groups, we screened out 152 salivary DEPs. We found orosomucoid 1(ORM1) had significantly higher expression in saliva of HCC patients compared with non-HCC groups (p < 0.001) and the expression of ORM1 in liver cancer tissues was significantly higher than that in adjacent normal tissues (p < 0.001). The combination of salivary ORM1 and alpha-fetoprotein (AFP) showed reasonable specificities and sensitivities for detecting HCC. In a word, salivary ORM1 as a new biomarker of hepatitis B associated hepatocellular carcinoma, combination of salivary ORM1 and AFP as an improved diagnostic tool for hepatocellular carcinoma.
引用
收藏
页数:13
相关论文
共 41 条
[1]   Sample Treatment for Saliva Proteomics [J].
Amado, Francisco ;
Calheiros-Lobo, Maria Joao ;
Ferreira, Rita ;
Vitorino, Rui .
EMERGING SAMPLE TREATMENTS IN PROTEOMICS, 2019, 1073 :23-56
[2]   Alpha-1-acid glycoprotein as potential biomarker for alpha-fetoprotein-low hepatocellular carcinoma [J].
Bachtiar I. ;
Kheng V. ;
Wibowo G.A. ;
Gani R.A. ;
Hasan I. ;
Sanityoso A. ;
Budhihusodo U. ;
Lelosutan S.A.R. ;
Martamala R. ;
Achwan W.A. ;
Soemoharjo S. ;
Sulaiman A. ;
Lesmana L.A. ;
Tai S. .
BMC Research Notes, 3 (1)
[3]   Combination of alpha-1-acid glycoprotein and alpha-fetoprotein as an improved diagnostic tool for hepatocellular carcinoma [J].
Bachtiar, Indra ;
Santoso, Julian Mulya ;
Atmanegara, Benny ;
Gani, Rino Alvani ;
Hasan, Irsan ;
Lesmana, Laurentius Adrianto ;
Sulaiman, Ali ;
Gu, Jianren ;
Tai, Susan .
CLINICA CHIMICA ACTA, 2009, 399 (1-2) :97-101
[4]   AFP promotes HCC progression by suppressing the HuR-mediated Fas/FADD apoptotic pathway [J].
Chen, Tianke ;
Dai, Xiaowei ;
Dai, Juji ;
Ding, Chaodong ;
Zhang, Zheng ;
Lin, Ziqi ;
Hu, Jin ;
Lu, Mei ;
Wang, Zhanyu ;
Qi, Yalei ;
Zhang, Li ;
Pan, Rulu ;
Zhao, Zhu ;
Lu, Liting ;
Liao, Wanqin ;
Lu, Xincheng .
CELL DEATH & DISEASE, 2020, 11 (10)
[5]   Ultrasound screening for hepatocellular carcinoma in patients with advanced liver fibrosis. An overview [J].
Danila, Mirela ;
Sporea, Ioan .
MEDICAL ULTRASONOGRAPHY, 2014, 16 (02) :139-144
[6]   iTRAQ-based proteomics reveals SOD2 as a potential salivary biomarker in liver cancer [J].
Ding, Feng ;
Sun, Kehuan ;
Sun, Ningning ;
Jiang, Qianqian ;
Cao, Meiqun ;
Wu, Zhengzhi .
INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS, 2019, 34 (03) :221-231
[7]   Combination of inflammatory score/liver function and AFP improves the diagnostic accuracy of HBV-related hepatocellular carcinoma [J].
Ding, Yezhou ;
Liu, Kehui ;
Xu, Yumin ;
Zhao, Qingqing ;
Lou, Shike ;
Xiang, Xiaogang ;
Yan, Lei ;
Cao, Zhujun ;
Xie, Qing ;
Zhu, Chuanwu ;
Bao, Shisan ;
Wang, Hui .
CANCER MEDICINE, 2020, 9 (09) :3057-3069
[8]   Trends in survival of patients with hepatocellular carcinoma between 1977 and 1996 in the United States [J].
El-Serag, HB ;
Mason, AC ;
Key, C .
HEPATOLOGY, 2001, 33 (01) :62-65
[9]   Biology and significance of alpha-fetoprotein in hepatocellular carcinoma [J].
Galle, Peter R. ;
Foerster, Friedrich ;
Kudo, Masatoshi ;
Chan, Stephen L. ;
Llovet, Josep M. ;
Qin, Shukui ;
Schelman, William R. ;
Chintharlapalli, Sudhakar ;
Abada, Paolo B. ;
Sherman, Morris ;
Zhu, Andrew X. .
LIVER INTERNATIONAL, 2019, 39 (12) :2214-2229
[10]  
Gani RA, 2015, ACTA MED INDONES, V47, P216