Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

被引：27
作者：
Gambis, Alexis [1 ]
Dourlen, Pierre [2 ]
Steller, Hermann [1 ]
Mollereau, Bertrand [2 ]
机构：
[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Apoptosis & Canc, New York, NY 10065 USA
[2] Univ Lyon, IFR Biosci Lyon Gerland 128, Ecole Normale Super Lyon, CNRS,Lab Mol Biol Cell,UMR5239, Lyon, France
来源：
DEVELOPMENTAL BIOLOGY | 2011年 / 351卷 / 01期
关键词：
Apoptosis; Neuron; Development; Polarity; Retina; CELL-DEATH; PLANAR POLARITY; FLUORESCENT PROTEIN; EYE; DIFFERENTIATION; ESTABLISHMENT; DEGENERATION; SCREEN; GENES; ROLES;
D O I：
10.1016/j.ydbio.2010.12.040
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (Pits) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：128 / 134
页数：7
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