We have studied the effect of the measurement system color temperature change on MTF both in theory and by experiment. Firstly, we simulated sorts of white light sources by computer and constructed the source spectrum characterizations of different color temperature. The simulated calculation results showed that in white light illumination condition, the value of MTF changed little even if color temperature change scope extend to 700K. In experiment we used "The digital optical transfer-function measuring equipment", an instrument we invented recently. We can see from the experimental data that the measurement results remained quite stable even if the voltage changed in wide range.