Tong-fu-li-fei decoction exerts a protective effect on intestinal barrier of sepsis in rats through upregulating ZO-1/occludin/claudin-1 expression

被引：53

作者：

Chen, Li ^{[1
]}

Li, Lan ^{[1
]}

Han, Yue ^{[1
]}

Lv, Bo ^{[1
]}

Zou, Suzhao ^{[1
]}

Yu, Qian ^{[1
]}

机构：

[1] Guizhou Univ Tradit Chinese Med, Affiliated Hosp 1, 71 Baoshan North Rd, Guiyang, Guizhou, Peoples R China

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2020年 / 143卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Tong-fu-li-fei decoction; Sepsis; Inflammation; Intestinal barrier; SEPTIC SHOCK;

D O I：

10.1016/j.jphs.2020.02.009

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Tong-fu-li-fei (TFL) prescription has already used to treat sepsis in clinic but its mechanism remains unclear. Here, we aimed to investigate the effect and mechanism of Tong-fu-li-fei (TFL) prescription on sepsis in rats. The Sprague-Dawley rats were divided into the sham group, model group, the TFL 3.6 g/kg and 7.2 g/kg-treated group. The sepsis model was induced by cecal ligation and puncture (CLP). After 7 days, TFL treatment improved the survival rate of CLP rats and alleviated sepsis-induced intestinal mucosal injury. The ELISA assay showed that inflammatory cytokine expressions including TNF-alpha and IL-1 beta in serum from TFL-treated rats were lower than that in the model. And TNF-alpha, IL-1 beta and IL-6 from intestinal tissues were also decreased and IL-10 was increased in TFL-treated rats. Meanwhile the expression levels of the tight junction molecules occludin, claudin-1, and zonula occludens-1 (ZO-1) mRNA and protein expressions examined by RT-PCR, western blot and immunohistochemistry, were also restored in rats that received TFL treatment. Our data showed that TFL mitigates the inflammatory response and maintains intestinal barrier function in sepsis through upregulating ZO-1/occludin/claudin-1 expression, providing a good experimental basis for its clinical treatment of sepsis. (C) 2020 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.

引用

页码：89 / 96

页数：8

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