Lysosomal Membrane Stability of the Mussel, Mytilus galloprovincialis (L.), as a Biomarker of Tributyltin Exposure

被引：11

作者：

Okoro, Hussein K. ^{[1
]}

Snyman, Reinette G. ^{[3
]}

Fatoki, Olalekan S. ^{[1
]}

Adekola, Folahan A. ^{[2
]}

Ximba, Bhekumusa J. ^{[1
]}

Slabber, Michelle Y. ^{[3
]}

机构：

[1] Cape Peninsula Univ Technol, Fac Sci Appl, Dept Chem, ZA-8000 Cape Town, South Africa

[2] Univ Ilorin, Fac Sci, Dept Chem, Ilorin, Nigeria

[3] Cape Peninsula Univ Technol, Fac Sci Appl, Dept Biodivers & Conservat Management, ZA-8000 Cape Town, South Africa

来源：

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY | 2015年 / 94卷 / 05期

关键词：

Tributyltin; Mussel; Neutral red retention time; Lysosomal membrane stability; Biomarker; ORGANOTIN COMPOUNDS; DIGESTIVE CELLS; CONTAMINATION; ENVIRONMENT; RESPONSES; BLOOD; BIOACCUMULATION; POLLUTION; SEAWATER; METALS;

D O I：

10.1007/s00128-015-1526-4

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The effect of tributyltin (TBT) on the stability of hemocytic lysosome membranes of the mussel, Mytilus galloprovincialis, and the use thereof as a biomarker of TBT-induced stress, was investigated. Mussels were exposed to 0.1 and 1.0 A mu g/L tributyltin respectively for 4 weeks. Lysosomal membrane stability of hemocytes was tested weekly by means of the neutral red retention time (NRRT) assay, after which the mussel samples were analyzed for TBT content. The two exposed groups exhibited significantly increased (p < 0.05) whole body TBT concentrations with concomitant significant decreases (p < 0.05) in NRRT (R-2 values of 0.85 and 0.971 for lower and higher exposure groups, respectively). The higher exposure group showed a typical dose-response curve. For the control, no TBT was detected and NRRT remained stable. It was concluded that the NRRT assay could be considered as a useful technique, and lysosomal membrane destabilization a useful early warning and cellular biomarker of stress due to TBT exposure in M. galloprovincialis.

引用

页码：609 / 613

页数：5

共 36 条

[1] An ecotoxicological approach for the Boka Kotorska Bay (south-eastern Adriatic Sea): First evaluation of lysosomal responses and metallothionein induction in mussels [J].

Da Ros, L. ;

Moschino, V. ;

Macic, V. ;

Schintu, M. .

MARINE POLLUTION BULLETIN, 2011, 63 (5-12) :326-333

[2] Evaluation of neutral red retention assay, micronucleus test, acetylcholinesterase activity and a signal transduction molecule (cAMP) in tissues of Mytilus galloprovincialis (L.), in pollution monitoring [J].

Dailianis, S ;

Domouhtsidou, GP ;

Raftopoulou, E ;

Kaloyianni, M ;

Dimitriadis, VK .

MARINE ENVIRONMENTAL RESEARCH, 2003, 56 (04) :443-470

[3] Relative sensitivity of two marine bivalves for detection of genotoxic and cytotoxic effects: a field assessment in the Tamar Estuary, South West England [J].

Dallas, Lorna J. ;

Cheung, Victoria V. ;

Fisher, Andrew S. ;

Jha, Awadhesh N. .

ENVIRONMENTAL MONITORING AND ASSESSMENT, 2013, 185 (04) :3397-3412

[4] Bioaccumulation of metals in black mussels (Mytilus galloprovincialis) in Cape Town Harbour, South Africa [J].

Olalekan S. Fatoki ;

Hussein K. Okoro ;

Folahan A. Adekola ;

Bhekumusa J. Ximba ;

Reinette G. Snyman .

The Environmentalist, 2012, 32 (1) :48-57

[5] Impact of the Sea Empress oil spill on lysosomal stability in mussel blood cells [J].

Fernley, PW ;

Moore, MN ;

Lowe, DM ;

Donkin, P ;

Evans, S .

MARINE ENVIRONMENTAL RESEARCH, 2000, 50 (1-5) :451-455

[6] Structural and functional changes in gill mitochondrial membranes from the Mediterranean mussel Mytilus galloprovincialis exposed to tri-n-butyltin [J].

Fiorini, Rosamaria ;

Pagliarani, Alessandra ;

Nesci, Salvatore ;

Pirini, Maurizio ;

Tucci, Elisabetta ;

Ventrella, Vittoria .

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2012, 31 (04) :877-884

[7] Organotin compounds in seawater and Mytilus galloprovincialis mussels along the Croatian Adriatic Coast [J].

Furdek, Martina ;

Vahcic, Mitja ;

Scancar, Janez ;

Milacic, Radmila ;

Kniewald, Goran ;

Mikac, Nevenka .

MARINE POLLUTION BULLETIN, 2012, 64 (02) :189-199

[8] Effects of tributyltin and benzo[a]pyrene on the immune-associated activities of hemocytes and recovery responses in the gastropod abalone, Haliotis diversicolor [J].

Gopalakrishnan, Singaram ;

Huang, Wei-Bin ;

Wang, Qiang-Wei ;

Wu, Man-Li ;

Liu, Jie ;

Wang, Ke-Jian .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2011, 154 (02) :120-128

[9] Toxicity of tributyltin in the marine mollusc Mytilus edulis [J].

Hagger, JA ;

Depledge, MH ;

Galloway, TS .

MARINE POLLUTION BULLETIN, 2005, 51 (8-12) :811-816

[10] Organotin compounds in the environment - an overview [J].

Hoch, M .

APPLIED GEOCHEMISTRY, 2001, 16 (7-8) :719-743

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