Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells

被引：227

作者：

Pan, Y. ^{[1
]}

Breidt, F., Jr. ^{[1
]}

机构：

[1] N Carolina State Univ, USDA ARS, Dept Microbiol, N Carolina Agr Res Serv, Raleigh, NC 27695 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 24期

关键词：

D O I：

10.1128/AEM.01198-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.

引用

页码：8028 / 8031

页数：4

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