RETRACTED: Propofol Suppresses Proliferation, Migration, Invasion And Promotes Apoptosis By Upregulating microRNA-140-5p In Gastric Cancer Cells (Retracted Article)

被引:12
作者
Zhu, Fengbo [1 ]
Li, Qiuxia [1 ]
Yang, Ying [2 ]
Wang, Liangui [1 ]
Wang, Jing [3 ]
机构
[1] Jinan Fifth Peoples Hosp, Dept Gastroenterol, Jinan 250022, Shandong, Peoples R China
[2] Jinan Fifth Peoples Hosp, Dept Hyperbar Oxygen, Jinan 250022, Shandong, Peoples R China
[3] Jinan Fifth Peoples Hosp, Dept Anesthesiol, 24297 Jingshi Rd, Jinan 250022, Shandong, Peoples R China
关键词
propofol; gastric cancer; miR-140-5p; migration; proliferation; apoptosis; INHIBITS PROLIFERATION; EXPRESSION;
D O I
10.2147/OTT.S225360
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Purpose: This study aimed to investigate the anti-tumor effect of propofol on gastric cancer (GC) and its underlying mechanisms. Patients and methods: SGC-7901 and MKN45 cells were transfected and divided into the following groups: Control group, Propofol group, Propofol+miR-140-5p inhibitor group and miR-140-5p inhibitor group. Moreover, cell proliferation, apoptosis, migration and invasion of SGC-7901 and MKN45 cells were evaluated by BrdU incorporation assay, Annexin V-FITC/PI double staining assay, wound healing assay and transwell assay, respectively. The mRNA expressions of matrix metalloproteinase 2 (MMP-2) and MMP-9 were detected by qRT-PCR. Cleaved caspase-3, Bcl-2, MMP-2 and MMP-9 expressions were detected by Western blot. Results: Propofol inhibited cell proliferation, migration and invasion, but promoted cell apoptosis in SGC-7901 and MKN45 cells. Propofol also elevated the expression of miR-140-5p. Suppression of miR-140-5p could reverse the effects of propofol on the biological behavior of SGC-7901 and MKN45 cells. Meanwhile, propofol treatment increased the expression of cleaved caspase-3 but decreased Bcl-2, MMP-2 and MMP-9 in SGC-7901 and MKN45 cells. The expression of cleaved caspase-3 was downregulated while Bcl-2, MMP-2 and MMP-9 were upregulated by miR-140-5p suppression. Conclusion: Propofol could inhibit cell proliferation, migration and invasion, as well as promote cell apoptosis by upregulating miR-140-5p in gastric cancer cells.
引用
收藏
页码:10129 / 10138
页数:10
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