Isolation and characterisation of wheat cDNA clones encoding PR4 proteins

被引:28
作者
Caruso, C [1 ]
Bertini, L
Tucci, M
Caporale, C
Leonardi, L
Saccardo, F
Bressan, RA
Veronese, P
Buonocore, V
机构
[1] Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy
[2] Univ Tuscia, Dipartimento Prod Vegetale, I-01100 Viterbo, Italy
[3] Purdue Univ, Dept Hort, Ctr Plant Environm Stress Physiol, W Lafayette, IN 47907 USA
来源
DNA SEQUENCE | 1999年 / 10卷 / 4-5期
关键词
fungal infection; Fusarium culmorum; pathogenesis-related protein; plant defense; PR4; genes; Triticum aestivum;
D O I
10.3109/10425179909033956
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two cDNA clones encoding the previously characterised PR4 proteins wheatwin 1 and wheatwin2 from wheat (Triticum aestivum cv. S. Pastore) have been identified and named wPR4a and wPR4b, respectively. The clones have been isolated by screening a cDNA library with a specific cDNA probe obtained by RT-PCR. The wPR4a and wPR4b cDNAs contain open reading frames of 441 and 447 bp that encode for wheatwin1 and wheatwin2, respectively.
引用
收藏
页码:301 / 307
页数:7
相关论文
共 31 条
[1]  
[Anonymous], 1987, Plant Molecular Biology Reporter
[2]   INTRACELLULAR TRAFFICKING OF SECRETORY PROTEINS [J].
BEDNAREK, SY ;
RAIKHEL, NV .
PLANT MOLECULAR BIOLOGY, 1992, 20 (01) :133-150
[3]   PLANT PATHOGENESIS-RELATED PROTEINS INDUCED BY VIRUS-INFECTION [J].
BOL, JF ;
LINTHORST, HJM ;
CORNELISSEN, BJC .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1990, 28 :113-138
[4]  
BOLLER T, 1987, PLANT MICROBE INTERA, V2, P385
[5]  
BOWLES DJ, 1990, ANNU REV BIOCHEM, V59, P873, DOI 10.1146/annurev.bi.59.070190.004301
[6]   WOUND-INDUCED ACCUMULATION OF MESSENGER-RNA CONTAINING A HEVEIN SEQUENCE IN LATICIFERS OF RUBBER TREE (HEVEA-BRASILIENSIS) [J].
BROEKAERT, W ;
LEE, HI ;
KUSH, A ;
CHUA, NH ;
RAIKHEL, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7633-7637
[7]  
Carr J. P., 1989, Genetic engineering: principles and methods. Volume 11., P65
[8]   THE AMINO-ACID-SEQUENCE OF A PROTEIN FROM WHEAT KERNEL CLOSELY-RELATED TO PROTEINS INVOLVED IN THE MECHANISMS OF PLANT DEFENSE [J].
CARUSO, C ;
CAPORALE, C ;
POERIO, E ;
FACCHIANO, A ;
BUONOCORE, V .
JOURNAL OF PROTEIN CHEMISTRY, 1993, 12 (04) :379-386
[9]   Structural and antifungal properties of a pathogenesis-related protein from wheat kernel [J].
Caruso, C ;
Caporale, C ;
Chilosi, G ;
Vacca, F ;
Bertini, L ;
Magro, P ;
Poerio, E ;
Buonocore, V .
JOURNAL OF PROTEIN CHEMISTRY, 1996, 15 (01) :35-44
[10]   Induction of pathogenesis-related proteins in germinating wheat seeds infected with Fusarium culmorum [J].
Caruso, C ;
Chilosi, G ;
Caporale, C ;
Leonardi, L ;
Bertini, L ;
Magro, P ;
Buonocore, V .
PLANT SCIENCE, 1999, 140 (01) :87-97