Comparison of PCR protocols including positive controls for detection of Chlamydia pneumoniae in respiratory specimens

被引：3

作者：

Metogho, RM ^{[1
]}

Deslandes, S ^{[1
]}

Frost, EH ^{[1
]}

机构：

[1] Univ Sherbrooke, Fac Med, Dept Microbiol, Sherbrooke, PQ J1K 2R1, Canada

来源：

MOLECULAR AND CELLULAR PROBES | 1999年 / 13卷 / 01期

关键词：

Chlamydia pneumoniae; PCR method; nucleic acid diagnosis; respiratory specimen; positive controls;

D O I：

10.1006/mcpr.1998.0217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to develop a practical approach for detection of Chlamydia pneumoniae by polymerase chain reaction (PCR) in clinical respiratory samples, positive controls for rDNA and major outer-membrane protein gene targets were constructed. Two PCR strategies detected chlamydial DNA in excess of 1000 copies per mi in the same 19 of 135 clinical specimens and identified PCR inhibitors in the same four samples. (C) 1999 Academic Press.

引用

页码：71 / 75

页数：5

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