Upregulation and hypomethylation of lncRNA AFAP1-AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer

被引：60
作者：
Bo, Hao [1 ,2 ,3 ,4 ]
Fan, Liqing [4 ]
Gong, Zhaojian [5 ]
Liu, Zhizhong [6 ,7 ]
Shi, Lei [2 ,3 ]
Guo, Can [2 ,3 ]
Li, Xiayu [2 ,3 ]
Liao, Qianjin [2 ,3 ,6 ,7 ]
Zhang, Wenling [2 ,3 ]
Zhou, Ming [1 ,2 ,3 ]
Xiang, Bo [1 ,2 ,3 ]
Li, Xiaoling [1 ,2 ,3 ]
Li, Guiyuan [1 ,2 ,3 ]
Xiong, Wei [1 ,2 ,3 ]
Zeng, Zhaoyang [1 ,2 ,3 ]
Cao, Ke [8 ]
Zhang, Shanshan [1 ,2 ,3 ]
Xiong, Fang [1 ,2 ,3 ]
机构：
[1] Cent South Univ, Xiangya Hosp, Key Lab Carcinogenesis, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410000, Hunan, Peoples R China
[2] Cent South Univ, Key Lab Carcinogenesis & Canc Invas, Chinese Minist Educ, Canc Res Inst, Changsha 410000, Hunan, Peoples R China
[3] Cent South Univ, Sch Basic Med Sci, Changsha 410000, Hunan, Peoples R China
[4] Cent South Univ, Inst Reprod & Stem Cell Engn, Sch Basic Med Sci, Changsha 410000, Hunan, Peoples R China
[5] Cent South Univ, Xiangya Hosp 2, Dept Oral & Maxillofacial Surg, Changsha 410000, Hunan, Peoples R China
[6] Cent South Univ, Hunan Key Lab Translat Radiat Oncol, Hunan Canc Hosp, Changsha 410000, Hunan, Peoples R China
[7] Cent South Univ, Affiliated Canc Hosp, Xiangya Sch Med, Changsha 410000, Hunan, Peoples R China
[8] Cent South Univ, Dept Oncol, Xiangya Hosp 3, Hunan Key Lab Nonresolving Inflammat & Canc, Changsha 410000, Hunan, Peoples R China
来源：
ONCOLOGY REPORTS | 2019年 / 41卷 / 04期
基金：
中国国家自然科学基金;
关键词：
long non-coding RNAs; AFAP1 antisense RNA1; cervical cancer; prognosis; migration and invasion; LONG NONCODING RNA; VIRUS-ENCODED MICRORNAS; NASOPHARYNGEAL CARCINOMA; GENE-EXPRESSION; CELL-PROLIFERATION; CIRCULAR RNAS; TUMOR-GROWTH; PROGRESSION; METASTASIS; DATABASE;
D O I：
10.3892/or.2019.7027
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Although lncRNA AFAP1 antisense RNA1 (AFAP1-AS1) is considered an oncogenic lncRNA, little is known about the role of AFAP1-AS1 in cervical cancer. In the present study, we found that AFAP1-AS1 was elevated and hypomethylated in cervical cancer and was associated with a poor prognosis of patients with cervical cancer by analyzing the Cancer RNA-Seq Nexus (CRN), MethCH and UCSC XENA databases. Subsequently, we knocked AFAP1-AS1 expression down using siRNAs in cervical cancer cells. Wound healing experiments and matrigel invasion experiments revealed that the downregulation of AFAP1-AS1 suppressed the migration and invasion of cervical cancer cells. Furthermore, western blot analysis demonstrated that the antitumor effects induced by the silencing of AFAP1-AS1 were mainly mediated through the regulation of the Rho/Rac signaling pathway and epithelial-mesenchymal transition (EMT)-related genes. Taken together, the findings of the present study indicate that the expression level of AFAP1-AS1 may be involved in the development of cervical cancer. Thus, AFAP1-AS1 may be a novel prognostic biomarker and a potential therapeutic target for patients with cervical cancer.
引用
收藏
页码：2431 / 2439
页数：9
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