Upregulation and hypomethylation of lncRNA AFAP1-AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer

被引：60

作者：

Bo, Hao ^{[1
,2
,3
,4
]}

Fan, Liqing ^{[4
]}

Gong, Zhaojian ^{[5
]}

Liu, Zhizhong ^{[6
,7
]}

Shi, Lei ^{[2
,3
]}

Guo, Can ^{[2
,3
]}

Li, Xiayu ^{[2
,3
]}

Liao, Qianjin ^{[2
,3
,6
,7
]}

Zhang, Wenling ^{[2
,3
]}

Zhou, Ming ^{[1
,2
,3
]}

Xiang, Bo ^{[1
,2
,3
]}

Li, Xiaoling ^{[1
,2
,3
]}

Li, Guiyuan ^{[1
,2
,3
]}

Xiong, Wei ^{[1
,2
,3
]}

Zeng, Zhaoyang ^{[1
,2
,3
]}

Cao, Ke ^{[8
]}

Zhang, Shanshan ^{[1
,2
,3
]}

Xiong, Fang ^{[1
,2
,3
]}

机构：

[1] Cent South Univ, Xiangya Hosp, Key Lab Carcinogenesis, Chinese Minist Hlth, 87 Xiangya Rd, Changsha 410000, Hunan, Peoples R China

[2] Cent South Univ, Key Lab Carcinogenesis & Canc Invas, Chinese Minist Educ, Canc Res Inst, Changsha 410000, Hunan, Peoples R China

[3] Cent South Univ, Sch Basic Med Sci, Changsha 410000, Hunan, Peoples R China

[4] Cent South Univ, Inst Reprod & Stem Cell Engn, Sch Basic Med Sci, Changsha 410000, Hunan, Peoples R China

[5] Cent South Univ, Xiangya Hosp 2, Dept Oral & Maxillofacial Surg, Changsha 410000, Hunan, Peoples R China

[6] Cent South Univ, Hunan Key Lab Translat Radiat Oncol, Hunan Canc Hosp, Changsha 410000, Hunan, Peoples R China

[7] Cent South Univ, Affiliated Canc Hosp, Xiangya Sch Med, Changsha 410000, Hunan, Peoples R China

[8] Cent South Univ, Dept Oncol, Xiangya Hosp 3, Hunan Key Lab Nonresolving Inflammat & Canc, Changsha 410000, Hunan, Peoples R China

来源：

ONCOLOGY REPORTS | 2019年 / 41卷 / 04期

基金：

中国国家自然科学基金;

关键词：

long non-coding RNAs; AFAP1 antisense RNA1; cervical cancer; prognosis; migration and invasion; LONG NONCODING RNA; VIRUS-ENCODED MICRORNAS; NASOPHARYNGEAL CARCINOMA; GENE-EXPRESSION; CELL-PROLIFERATION; CIRCULAR RNAS; TUMOR-GROWTH; PROGRESSION; METASTASIS; DATABASE;

D O I：

10.3892/or.2019.7027

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Although lncRNA AFAP1 antisense RNA1 (AFAP1-AS1) is considered an oncogenic lncRNA, little is known about the role of AFAP1-AS1 in cervical cancer. In the present study, we found that AFAP1-AS1 was elevated and hypomethylated in cervical cancer and was associated with a poor prognosis of patients with cervical cancer by analyzing the Cancer RNA-Seq Nexus (CRN), MethCH and UCSC XENA databases. Subsequently, we knocked AFAP1-AS1 expression down using siRNAs in cervical cancer cells. Wound healing experiments and matrigel invasion experiments revealed that the downregulation of AFAP1-AS1 suppressed the migration and invasion of cervical cancer cells. Furthermore, western blot analysis demonstrated that the antitumor effects induced by the silencing of AFAP1-AS1 were mainly mediated through the regulation of the Rho/Rac signaling pathway and epithelial-mesenchymal transition (EMT)-related genes. Taken together, the findings of the present study indicate that the expression level of AFAP1-AS1 may be involved in the development of cervical cancer. Thus, AFAP1-AS1 may be a novel prognostic biomarker and a potential therapeutic target for patients with cervical cancer.

引用

页码：2431 / 2439

页数：9

共 69 条

[1]

[Anonymous], ONCOTARGET

[2] High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis [J].

Bo, Hao ;

Fan, Liqing ;

Li, Jingjing ;

Liu, Zhizhong ;

Zhang, Shanshan ;

Shi, Lei ;

Guo, Can ;

Li, Xiayu ;

Liao, Qianjin ;

Zhang, Wenling ;

Zhou, Ming ;

Xiang, Bo ;

Li, Xiaoling ;

Li, Guiyuan ;

Xiong, Wei ;

Zeng, Zhaoyang ;

Xiong, Fang ;

Gong, Zhaojian .

JOURNAL OF CANCER, 2018, 9 (24) :4677-4683

[3] Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma [J].

Bo, Hao ;

Gong, Zhaojian ;

Zhang, Wenling ;

Li, Xiayu ;

Zeng, Yong ;

Liao, Qianjin ;

Chen, Pan ;

Shi, Lei ;

Lian, Yu ;

Jing, Yizhou ;

Tang, Ke ;

Li, Zheng ;

Zhou, Yanhong ;

Zhou, Ming ;

Xiang, Bo ;

Li, Xiaoling ;

Yang, Jianbo ;

Xiong, Wei ;

Li, Guiyuan ;

Zeng, Zhaoyang .

ONCOTARGET, 2015, 6 (24) :20404-20418

[4] Integrated genomic and molecular characterization of cervical cancer [J].

Burk, Robert D. ;

Chen, Zigui ;

Saller, Charles ;

Tarvin, Katherine ;

Carvalho, Andre L. ;

Scapulatempo-Neto, Cristovam ;

Silveira, Henrique C. ;

Fregnani, Jose H. ;

Creighton, Chad J. ;

Anderson, Matthew L. ;

Castro, Patricia ;

Wang, Sophia S. ;

Yau, Christina ;

Benz, Christopher ;

Robertson, A. Gordon ;

Mungall, Karen ;

Lim, Lynette ;

Bowlby, Reanne ;

Sadeghi, Sara ;

Brooks, Denise ;

Sipahimalani, Payal ;

Mar, Richard ;

Ally, Adrian ;

Clarke, Amanda ;

Mungall, Andrew J. ;

Tam, Angela ;

Lee, Darlene ;

Chuah, Eric ;

Schein, Jacqueline E. ;

Tse, Kane ;

Kasaian, Katayoon ;

Ma, Yussanne ;

Marra, Marco A. ;

Mayo, Michael ;

Balasundaram, Miruna ;

Thiessen, Nina ;

Dhalla, Noreen ;

Carlsen, Rebecca ;

Moore, Richard A. ;

Holt, Robert A. ;

Jones, Steven J. M. ;

Wong, Tina ;

Pantazi, Angeliki ;

Parfenov, Michael ;

Kucherlapati, Raju ;

Hadjipanayis, Angela ;

Seidman, Jonathan ;

Kucherlapati, Melanie ;

Ren, Xiaojia ;

Xu, Andrew W. .

NATURE, 2017, 543 (7645) :378-+

[5] The up-regulation of long non-coding RNA AFAP1-AS1 is associated with the poor prognosis of NSCLC patients [J].

Deng, Jun ;

Liang, Yujia ;

Liu, Chunfeng ;

He, Shengdong ;

Wang, Songping .

BIOMEDICINE & PHARMACOTHERAPY, 2015, 75 :8-11

[6] Application of atomic force microscopy in cancer research [J].

Deng, Xiangying ;

Xiong, Fang ;

Li, Xiayu ;

Xiang, Bo ;

Li, Zheng ;

Wu, Xu ;

Guo, Can ;

Li, Xiaoling ;

Li, Yong ;

Li, Guiyuan ;

Xiong, Wei ;

Zeng, Zhaoyang .

JOURNAL OF NANOBIOTECHNOLOGY, 2018, 16 :V

[7] Long non-coding RNA LOC284454 promotes migration and invasion of nasopharyngeal carcinoma via modulating the Rho/Rac signaling pathway [J].

Fan, Chunmei ;

Tang, Yanyan ;

Wang, Jinpeng ;

Wang, Yian ;

Xiong, Fang ;

Zhang, Shanshan ;

Li, Xiayu ;

Xiang, Bo ;

Wu, Xu ;

Guo, Can ;

Ma, Jian ;

Zhou, Ming ;

Li, Xiaoling ;

Xiong, Wei ;

Li, Yong ;

Li, Guiyuan ;

Zeng, Zhaoyang .

CARCINOGENESIS, 2019, 40 (02) :380-391

[8] The emerging role of Epstein-Barr virus encoded microRNAs in nasopharyngeal carcinoma [J].

Fan, Chunmei ;

Tang, Yanyan ;

Wang, Jinpeng ;

Xiong, Fang ;

Guo, Can ;

Wang, Yumin ;

Xiang, Bo ;

Zhou, Ming ;

Li, Xiayu ;

Wu, Xu ;

Li, Yong ;

Li, Xiaoling ;

Li, Guiyuan ;

Xiong, Wei ;

Zeng, Zhaoyang .

JOURNAL OF CANCER, 2018, 9 (16) :2852-2864

[9] Role of long non-coding RNAs in glucose metabolism in cancer [J].

Fan, Chunmei ;

Tang, Yanyan ;

Wang, Jinpeng ;

Xiong, Fang ;

Guo, Can ;

Wang, Yumin ;

Zhang, Shanshan ;

Gong, Zhaojian ;

Wei, Fang ;

Yang, Liting ;

He, Yi ;

Zhou, Ming ;

Li, Xiaoling ;

Li, Guiyuan ;

Xiong, Wei ;

Zeng, Zhaoyang .

MOLECULAR CANCER, 2017, 16

[10] An integrative transcriptomic analysis reveals p53 regulated miRNA, mRNA, and lncRNA networks in nasopharyngeal carcinoma [J].

Gong, Zhaojian ;

Yang, Qian ;

Zeng, Zhaoyang ;

Zhang, Wenling ;

Li, Xiayu ;

Zu, Xuyu ;

Deng, Hao ;

Chen, Pan ;

Liao, Qianjin ;

Xiang, Bo ;

Zhou, Ming ;

Li, Xiaoling ;

Li, Yong ;

Xiong, Wei ;

Li, Guiyuan .

TUMOR BIOLOGY, 2016, 37 (03) :3683-3695

← 1 2 3 4 5 6 7 →