Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction

被引:52
作者
Britto, C
Silveira, C
Cardoso, MA
Marques, P
Luquetti, A
Macêdo, V
Fernandes, O
机构
[1] FIOCRUZ, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, BR-21045900 Rio De Janeiro, Brazil
[2] FIOCRUZ, Inst Oswaldo Cruz, Dept Trop Med, BR-21045900 Rio De Janeiro, Brazil
[3] Univ Brasilia, Dept Trop Med, Brasilia, DF, Brazil
[4] Univ Fed Goias, Inst Patol Trop & Saude Publ, Goiania, Go, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2001年 / 96卷 / 06期
关键词
Trypanosoma cruzi; kinetoplast DNA; Chagas disease; PCR; treatment;
D O I
10.1590/S0074-02762001000600014
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to FCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.
引用
收藏
页码:823 / 826
页数:4
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