Differential function of lysophosphatidic acid receptors in cell proliferation and migration of neuroblastoma cells

被引：59

作者：

Hayashi, Mai ^{[1
]}

Okabe, Kyoko ^{[1
]}

Kato, Kohei ^{[1
]}

Okumura, Mai ^{[1
]}

Fukui, Rie ^{[1
]}

Fukushima, Nobuyuki ^{[2
]}

Tsujiuchi, Toshifumi ^{[1
]}

机构：

[1] Kinki Univ, Div Canc Biol & Bioinformat, Dept Life Sci, Fac Sci & Engn, Higashiosaka, Osaka 5778502, Japan

[2] Kinki Univ, Div Mol Neurobiol, Dept Life Sci, Fac Sci & Engn, Higashiosaka, Osaka 5778502, Japan

来源：

CANCER LETTERS | 2012年 / 316卷 / 01期

关键词：

LPA; LPA receptor; Neuroblastoma; B103; Rat; LPA RECEPTORS; GROWTH-FACTOR; EXPRESSION; GENE; CANCER; MUTATIONS;

D O I：

10.1016/j.canlet.2011.10.030

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Lysophosphatidic acid (LPA) is a bioactive lipid mediator that induces diverse cellular biological effects and interacts with G protein-coupled transmembrane LPA receptors. In the present study, to assess biological roles of LPA receptors in the pathogenesis of tumor cells, each LPA receptor (Lpar1, Lpar2 or Lpar3)-expressing rat neuroblastoma B103 cells (Ipa1-1, Ipa2-2 or Ipa3-3-2 cells, respectively) were used. In cell motility and invasion assay, Ipa2-2 and Ipa3-3-2 cells showed significant higher intrinsic activity without LPA treatment than LPA receptor-unexpressing AB2-1 bf cells. LPA treatment further increased cell motility of these cells, which was suppressed by the pretreatment with inhibitors of Gi, Gq protein, or ROCK. By contrast, Ipa1-1 cells markedly decreased intrinsic cell motility and invasion, compared with AB2-1 bf cells. Constitutively active mutant Lpar1-expressing cells (Ipa1 Delta-1) showed significant high motility, comparable with those of Ipa2-2 and Ipa3-3-2. In soft agar assay. Ipa3-3-2 and Ipa1 Delta-1 cells showed colony formation, but other cells failed. These results suggest that LPA receptors may play different roles in cell proliferation and migration of rat neuroblastoma cells. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：91 / 96

页数：6

共 28 条

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