Aldehydes can switch the chemoselectivity of electrophiles in protein labeling

被引：14

作者：

Adusumalli, Srinivasa Rao ^{[1
]}

Rawale, Dattatraya Gautam ^{[1
]}

Rai, Vishal ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res Bhopal, Dept Chem, Organ & Bioconjugate Chem Lab, Bhopal Bypass Rd, Bhopal 462066, India

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2018年 / 16卷 / 48期

关键词：

PEPTIDES; SURFACE; REACTIVITY; CYSTEINE; SITE;

D O I：

10.1039/c8ob02897d

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We show that the chemoselectivity of an electrophile in protein labeling can be promiscuous. An aldehyde enables switching of chemoselectivity of an epoxide and a sulfonate ester along with an enhanced rate of reaction. The chemical technology renders single-site installation of diverse probes on a protein and delivers analytically pure tagged proteins.

引用

收藏

页码：9377 / 9381

页数：5

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