Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s

被引：16
作者：
Wang, Yao [1 ]
Wang, Chen-Fei [2 ]
Lie, Ming [5 ]
Zhou, De-Zhong [2 ]
Huang, Wei [1 ]
Wang, Wen-Xin [1 ,3 ,4 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, State Key Lab Met Matrix Composites, Shanghai 200240, Peoples R China
[2] Xi An Jiao Tong Univ, Sch Chem Engn & Technol, Xian 710049, Peoples R China
[3] Univ Coll Dublin, Charles Inst Dermatol, Sch Med, Dublin 4, Ireland
[4] Univ Coll Dublin, Sch Mech & Mat Engn, Dublin 4, Ireland
[5] Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Dept Dermatol, Shanghai 200025, Peoples R China
来源：
CHINESE JOURNAL OF POLYMER SCIENCE | 2020年 / 38卷 / 08期
基金：
中国国家自然科学基金; 爱尔兰科学基金会;
关键词：
Gene therapy; Non-viral gene vector; Highly branched polymer; Branching strategy; POLYMER LIBRARY APPROACH; DELIVERY EFFICIENCY; AMINE GROUPS; DENDRIMERS; MOLECULE; OLIGOMER; THERAPY; CELLS;
D O I：
10.1007/s10118-020-2393-y
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Highly branched poly(beta-amino ester)s (HPAEs) have emerged as one type of the most viable non-viral gene delivery vectors, both in vitro and in vivo. However, the effects of different branching strategies on the gene transfection performance have not yet been explored. Here, using triacrylate (B3) and diamine (B4) as the branching monomers, a series of HPAEs were synthesized via the "A2 + B3 + C2" and "A2 + B4 + C2" strategies, respectively. Results show that the branching strategy plays a pivotal role in dictating the physiological properties of the HPAE/DNA polyplexes and thus leads to obviously different cell viability and transfection efficiency. Comparatively, HPAEs synthesized via the "A2 + B3 + C2" branching strategy are more favorable for DNA transfection than that synthesized via the "A2 + B4 + C2" strategy. This study may provide new insights into the development of HPAEs based non-viral DNA delivery system.
引用
收藏
页码：830 / 839
页数：10
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