Tissue-engineered skeletal muscle organoids for reversible gene therapy

被引：114

作者：

Vandenburgh, H

DelTatto, M

Shansky, J

Lemaire, J

Chang, A

Payumo, F

Lee, P

Goodyear, A

Raven, L

机构：

[1] BROWN UNIV,SCH MED,DEPT PATHOL,PROVIDENCE,RI 02906

[2] BROWN UNIV,DEPT MOL PHARMACOL & BIOTECHNOL,PROVIDENCE,RI 02906

来源：

HUMAN GENE THERAPY | 1996年 / 7卷 / 17期

关键词：

D O I：

10.1089/hum.1996.7.17-2195

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genetically modified murine skeletal myoblasts were tissue engineered in vitro into organ-like structures (organoids) containing only postmitotic myofibers secreting pharmacological levels of recombinant human growth hormone (rhGH). Subcutaneous organoid implantation under tension led to the rapid and stable appearance of physiological sera levels of rhGH for up to 12 weeks, whereas surgical removal led to its rapid disappearance. Reversible delivery of bioactive compounds from postmitotic cells in tissue engineered organs has several advantages over other forms of muscle gene therapy.

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页码：2195 / 2200

页数：6

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