Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells

被引:19
作者
Tonoike, Yurie [1 ,2 ]
Matsushita, Kazuyuki [1 ,3 ]
Tomonaga, Takeshi [3 ,4 ]
Katada, Koji [1 ,2 ]
Tanaka, Nobuko [1 ]
Shimada, Hideaki [5 ]
Nakatani, Yukio [6 ]
Okamoto, Yoshitaka [2 ]
Nomura, Fumio [1 ]
机构
[1] Chiba Univ Hosp, Dept Mol Diag, Grad Sch Med, Chiba 2608670, Japan
[2] Chiba Univ Hosp, Dept Otorhinolaryngol, Chiba 2608670, Japan
[3] Chiba Univ Hosp, Dept Clin Prote Res Ctr, Chiba 2608670, Japan
[4] Natl Inst Biomed Innovat, Proteome Res Ctr, Proteome Res Project, Ibaraki, Osaka 5670085, Japan
[5] Toho Univ, Omori Med Ctr, Dept Gen & Gastrointestinal Surg, Tokyo 1438541, Japan
[6] Chiba Univ Hosp, Dept Diagnost Pathol, Grad Sch Med, Chiba 2608670, Japan
来源
BMC CELL BIOLOGY | 2011年 / 12卷
关键词
EPITHELIAL-MESENCHYMAL-TRANSITION; MELANIN-CONCENTRATING HORMONE-RECEPTOR-1; ESOPHAGEAL CANCER; BINDING PROTEIN; CARCINOMA-CELLS; INVASION; KINASE; ACTIVATION; EXPRESSION; MIGRATION;
D O I
10.1186/1471-2121-12-41
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background: We previously reported that periplakin (PPL) is downregulated in human esophageal cancer tissues compared to the adjacent non-cancer epithelium. Thus PPL could be a useful marker for detection of early esophageal cancer and evaluation of tumor progression, but largely remains unknown in this field. To investigate PPL involvement in carcinogenesis, tumor progression, cellular movement or attachment activity, siRNAs against PPL were transfected into pharyngeal squamous cancer cell lines and their effects on cellular behaviours were examined. Results: PPL knockdown appeared to decrease tumor cell growth together with G2/M phase accumulation in cells attached to a culture dish. However, the extent of cell growth suppression, evaluated by the number of cells attached to the culture dish, was too distinctive to be explained only by cell cycle delay. Importantly, PPL knockdown suppressed cellular movement and attachment to the culture dish accompanied by decreased pAktSer473 phosphorylation. Additionally, LY294002, a PI3K inhibitor that dephosphorylates pAktSer473, significantly suppressed D562 cell migration. Thus PPL potentially engages in cellular movement al least partly via the PI3K/Akt axis. Conclusions: PPL knockdown is related to reduced cellular movement and attachment activity in association with PI3K/Akt axis suppression, rather than malignant progression in pharyngeal cancer cells.
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页数:10
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