Cloning and Expression of One Chloroplastic Ascorbate Peroxidase Gene from Nelumbo nucifera

被引：8
|
作者：
Dong, Chen [1 ]
Zheng, Xingfei [1 ]
Li, Guolin [1 ]
Pan, Cheng [1 ]
Zhou, Mingquan [2 ]
Hu, Zhongli [1 ]
机构：
[1] Wuhan Univ, Coll Life Sci, Minist Educ Plant Dev Biol, Key Lab, Wuhan 430072, Peoples R China
[2] Wuhan Univ, Lotus Ctr, Wuhan 430072, Peoples R China
来源：
BIOCHEMICAL GENETICS | 2011年 / 49卷 / 9-10期
基金：
中国国家自然科学基金;
关键词：
APX; MnSOD; Synergistic expression; SUPEROXIDE-DISMUTASE; MOLECULAR-CLONING; CDNA; STRESS;
D O I：
10.1007/s10528-011-9440-x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel ascorbate peroxidase (APX) cDNA was obtained from Nelumbo nucifera (Elian). The phylogenetic analysis indicated that N. nucifera APX grouped together with chloroplastic APX of high plants. The recombinant protein expressed by PET-30a vector showed APX activity (0.04 mM ascorbate min(-1) mg(-1) protein). The APX mRNA was expressed in young leaves, roots, terminal buds, and leaf-stalks. Synergistic expression of N. nucifera APX and MnSOD mRNA was indicated in the short-term response to mechanical wounding.
引用
收藏
页码：656 / 664
页数：9
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