In vitro evaluation of the inhibitory effect of canine serum, canine fresh frozen plasma, freeze-thaw-cycled plasma, and Solcoseryl™ on matrix metalloproteinases 2 and 9

ObjectiveCompare the efficacy of canine serum, fresh frozen plasma (FFP), freeze-thaw-cycled plasma (FTCP), and Solcoseryl at inhibiting matrix metalloproteinases (MMP) 2 and 9 in vitro. ProcedureMatrix metalloproteinases 2 and 9 activity in the presence of serum, FFP, FTCP, or Solcoseryl was assayed using a commercially available fluorogenic gelatinase activity kit. ResultsMatrix metalloproteinases 2 activity in the presence of serum, FFP, FTCP, and Solcoseryl was 20.84%, 5.76%, 8.10%, and 83.03%, respectively of uninhibited MMP 2 activity. MMP 9 activity in the presence of serum, FFP, FTCP, and Solcoseryl was 57.36%, 58.35%, 49.35%, and -8.69%, respectively of uninhibited MMP 9 activity. ConclusionSerum, FFP, and FTCP exhibit similar levels of MMP 2 and 9 inhibitions. Solcoseryl causes minimal MMP 2 inhibition, but profound MMP 9 inhibition.