The Retrograde IFT Machinery of C. elegans Cilia: Two IFT Dynein Complexes?

被引:39
作者
Hao, Limin [1 ]
Efimenko, Evgeni [2 ]
Swoboda, Peter [2 ]
Scholey, Jonathan M. [1 ]
机构
[1] Univ Calif Davis, Dept Mol & Cell Biol, Davis, CA 95616 USA
[2] Karolinska Inst, Novum, Dept Biosci & Nutr, Ctr Biosci, Huddinge, Sweden
基金
瑞典研究理事会; 美国国家卫生研究院;
关键词
NEMATODE CAENORHABDITIS-ELEGANS; INTRAFLAGELLAR TRANSPORT IFT; KINESIN-LIKE PROTEIN; SENSORY CILIA; CYTOPLASMIC DYNEIN; CHEMOSENSORY NEURONS; HEAVY-CHAIN; CHLAMYDOMONAS; MOTORS; GENES;
D O I
10.1371/journal.pone.0020995
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We analyzed the relatively poorly understood IFT-dynein (class DYNC2)-driven retrograde IFT pathway in C. elegans cilia, which yielded results that are surprising in the context of current models of IFT. Assays of C. elegans dynein gene expression and intraflagellar transport (IFT) suggest that conventional IFT-dynein contains essential heavy (CHE-3), light-intermediate (XBX-1), plus three light polypeptide chains that participate in IFT, but no "essential" intermediate chain. IFT assays of XBX1::YFP suggest that IFT-dynein is transported as cargo to the distal tip of the cilium by kinesin-2 motors, but independent of the IFT-particle/BBSome complexes. Finally, we were surprised to find that the subset of cilia present on the OLQ (outer labial quadrant) neurons assemble independently of conventional "CHE-3" IFT-dynein, implying that there is a second IFT-dynein acting in these cilia. We have found a novel gene encoding a dynein heavy chain, DHC-3, and two light chains, in OLQ neurons, which could constitute an IFT-dynein complex in OLQ neuronal cilia. Our results underscore several surprising features of retrograde IFT that require clarification.
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页数:9
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