Argyrophilic nucleolar organizer regions (AgNORs) in interphases and metaphases of normal and neoplastic gill cells of Macoma balthica (Bivalvia: Tellinidae) from the Gulf of Gdansk, Baltic Sea

被引:9
作者
Smolarz, K
Wolowicz, M
Thiriot-Quiévreux, C
机构
[1] UPMC, Ctr Natl Rech Sci, Observ Oceanol, INSU, F-06230 Villefranche Sur Mer, France
[2] Univ Gdansk, Inst Oceanog, Lab Estuarine Ecol, PL-81378 Gdynia, Poland
关键词
silver-staining; nucleolar organizer regions; neoplasia; Macoma balthica; Baltic Sea;
D O I
10.3354/dao056269
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Chromosome analysis of gill cells of different populations of Macoma balthica (L.) from the Bay of Gdansk (Baltic Sea) revealed 2 clam categories, 1 with neoplastic features and 1 without. Silver-staining was performed on interphase and metaphase cells of both categories. The mean argyrophilic nucleolar organizer region (AgNOR) count per abnormal interphase cell was significantly higher than in normal interphase cells. Normal silver-stained metaphases had 3 nucleolar organizer region (NOR) chromosome phenotypes. The location of the NORs in the most frequent phenotype (55.6% in 54 metaphases scored) was interstitial on the largest metacentric chromosome pair, Pair No. 1. Abnormal silver-stained metaphases had a higher number of active NOR sites. Different phenotypes were observed (frequency greater than 10% for 67 metaphases scored); 2 were similar to those in normal metaphases and 5 were ectopic. The higher activity of AgNORs observed in abnormal cells confirmed the diagnosis of malignant neoplasia.
引用
收藏
页码:269 / 274
页数:6
相关论文
共 28 条
[1]   Structural study of a possible neoplasia detected in Mytilus galloprovincialis collected from the Ria of Vigo (NW Spain) [J].
Alonso, A ;
Suarez, P ;
Alvarez, C ;
San Juan, F ;
Molist, P .
DISEASES OF AQUATIC ORGANISMS, 2001, 47 (01) :73-79
[2]   Ectopic nucleolar organizer regions - A common anomaly revealed by Ag-NOR staining of metaphases from nine cancers [J].
Atkin, NB ;
Baker, MC .
CANCER GENETICS AND CYTOGENETICS, 1995, 85 (02) :129-132
[3]   Characterization of multiple 12p rearrangements in testicular germ cell tumor cell line 833K and its subclone 64CP by chromosome microdissection [J].
Blough, RI ;
Vance, GH ;
Henegariu, O ;
Smolarek, TA ;
Sledge, GW ;
Heerema, NA .
CANCER GENETICS AND CYTOGENETICS, 1998, 106 (01) :24-29
[4]   ECTOPIC NUCLEOLUS ORGANIZER REGIONS (NORS) IN HUMAN TESTICULAR-TUMORS [J].
DELOZIERBLANCHET, CD ;
WALT, H ;
ENGEL, E .
CYTOGENETICS AND CELL GENETICS, 1986, 41 (02) :107-113
[5]   The AgNORs [J].
Derenzini, M .
MICRON, 2000, 31 (02) :117-120
[6]  
DIXON DR, 1982, MAR BIOL LETT, V3, P163
[7]  
ELSTON RA, 1992, REV AQUAT SCI, V6, P405
[8]   VISUALIZATION OF RIBOSOMAL GENE ACTIVITY - SILVER STAINS PROTEINS ASSOCIATED WITH RIBOSOMAL-RNA TRANSCRIBED FROM OOCYTE CHROMOSOMES [J].
HOWELL, WM .
CHROMOSOMA, 1977, 62 (04) :361-367
[9]   CONTROLLED SILVER-STAINING OF NUCLEOLUS ORGANIZER REGIONS WITH A PROTECTIVE COLLOIDAL DEVELOPER - A 1-STEP METHOD [J].
HOWELL, WM ;
BLACK, DA .
EXPERIENTIA, 1980, 36 (08) :1014-1015
[10]   SILVER STAINING AS AN INDICATOR OF ACTIVE RIBOSOMAL GENES [J].
HUBBELL, HR .
STAIN TECHNOLOGY, 1985, 60 (05) :285-294