A graphene field effect capacitive Immunosensor for sub-femtomolar food toxin detection

被引：33
作者：
Basu, J. [1 ]
Datta, S. [1 ]
RoyChaudhuri, C. [1 ]
机构：
[1] Indian Inst Engn Sci & Technol, Dept Elect & Telecommun Engn, Sibpur 711103, Howrah, India
来源：
BIOSENSORS & BIOELECTRONICS | 2015年 / 68卷
关键词：
Reduced graphene oxide; Field effect capacitance; Quantum capacitance; Double layer capacitance; Food toxin detection; SPECTROSCOPY; TRANSISTORS; PLATFORM; FILMS; LAYER;
D O I：
10.1016/j.bios.2015.01.046
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this paper we report the sensing of aflatoxin B1(AFB1) by field effect capacitive method using electrophoretically deposited reduced graphene oxide (RGO) films for the first time. The RGO film has been characterized using SEM, surface profilometer and Raman spectroscopy. It has been observed that both quantum capacitance of RGO (C-q) and effective electrical double layer capacitance (C-EDL) contribute significantly towards the overall sensitivity for molar concentration in the range of 20-50 mM. As C-q and C-EDL changes in opposite direction after AFB1 capture and the nature of frequency dependence of C-q and C-EDL are different, the sensitivity shows a minima at a particular frequency. Interestingly, the sensitivity minima is also dependent on AFB1 concentration. Further, the maximum sensitivity obtained is around 30% for 10(-4) ppt (0.1 fg/ml) AFB1 which is greater than 1.5 times that of previous reports. This has been possible through the enhanced biomolecule immobilization capability of RGO. Thus the RGO based field effect capacitive sensor provides a combined advantage of both a high sensitivity and concentration dependent frequency behavior. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：544 / 549
页数：6
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