Electron transfer in organized assemblies of biomolecules.: Step-by-step avidin/biotin construction and dynamic characteristics of a spatially ordered multilayer enzyme electrode

被引：126

作者：

Anicet, N

Bourdillon, C

Moiroux, J

Savéant, JM

机构：

[1] Univ Paris 07, Electrochim Mol Lab, CNRS, Unite Mixte Rech Univ 7591, F-75251 Paris 05, France

[2] Univ Technol Compiegne, Technol Enzymat Lab, UPRESA 6022, F-60205 Compiegne, France

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 1998年 / 102卷 / 49期

关键词：

D O I：

10.1021/jp982352m

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The avidin-biotin technology may be used successfully to build stable and spatially ordered assemblies of monomolecular enzyme layers on surfaces as illustrated with the example of glucose oxidase and glassy carbon electrodes. With ferrocene methanol as the cosubstrate, cyclic voltammetry allows a detailed analysis of the catalytic responses, thus providing a demonstration of the spatial order of the multilayer structure and an estimate of the average distance between monomolecular layers. The respective advantages of the avidin-biotin and antigen-antibody technologies are discussed.

引用

页码：9844 / 9849

页数：6

共 36 条

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ANICET A, IN PRESS J AM CHEM S

[2]

ANNE A, IN PRESS LANGMUIR

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