Microfluidics-Based Assessment of Cell Deformability

被引：152

作者：

Adamo, Andrea ^{[1
]}

Sharei, Armon ^{[1
]}

Adamo, Luigi ^{[2
]}

Lee, ByungKun ^{[3
]}

Mao, Shirley ^{[1
]}

Jensen, Klavs F. ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] Washington Univ, Dept Med, Sch Med St Louis, St Louis, MO 63110 USA

[3] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 15期

关键词：

ATOMIC-FORCE MICROSCOPY; CANCER-CELLS; BIOLOGICAL-MATERIALS; FLOW CYTOMETER; LIVING CELLS; MECHANICS; BIOMECHANICS; SPECTROSCOPY; DISEASES;

D O I：

10.1021/ac300264v

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mechanical properties of cells have been shown to have a significant role in disease, as in many instances cell stiffness changes when a cell is no longer healthy. We present a high-throughput microfluidics-based approach that exploits the connection between travel time of a cell through a narrow passage and cell stiffness. The system resolves both cell travel time and relative cell diameter while retaining information on the cell level. We show that stiffer cells have longer transit times than less stiff ones and that cell size significantly influences travel times. Experiments with untreated HeLa cells and cells made compliant with latrunculin A and cytochalasin B further demonstrate that travel time is influenced by cell stiffness, with the compliant cells having faster transit time.

引用

页码：6438 / 6443

页数：6

共 25 条

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