Highly purified CD38+ sub-populations show no evidence of preferential clonal evolution despite having increased proliferative activity when compared with CD38- sub-populations derived from the same chronic lymphocytic leukaemia patient

被引:16
作者
Lin, Thet Thet [1 ]
Hewamana, Saman [1 ]
Ward, Rachel [1 ]
Taylor, Hannah [2 ]
Payne, Tammy [2 ]
Pratt, Guy [3 ]
Baird, Duncan [4 ]
Fegan, Chris [1 ]
Pepper, Chris [1 ]
机构
[1] Cardiff Univ, Sch Med, Dept Haematol, Cardiff CF14 4XN, S Glam, Wales
[2] Llandough Hosp, Dept Haematol, Penarth, S Glam, Wales
[3] Birmingham Heartlands Hosp, Dept Haematol, Birmingham, W Midlands, England
[4] Cardiff Univ, Sch Med, Dept Pathol, Cardiff CF14 4XN, S Glam, Wales
关键词
chronic lymphocytic leukaemia; CD38; telomere length; telomerase; FISH analysis;
D O I
10.1111/j.1365-2141.2008.07236.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In agreement with a recently published manuscript, this present study demonstrated that CD38(+) sub-populations had increased proliferative activity as evidenced by higher Ki-67 expression (P < 0.0001). This raised the possibility that the CD38(+) fraction is exposed to an increased risk of clonal evolution. However, serial fluorescence in situ hybridisation analysis of highly purified CD38(+) and CD38(-) sub-populations from individual patients revealed no distinct cytogenetic lesions or evidence of preferential clonal evolution in the CD38(+) fractions when compared with their CD38(-) counter-parts (P = 0.13). Furthermore, telomere length analysis revealed that all of the sub-populations had similarly short telomeres (P = 0.31) and comparably low telomerase (TERT) expression (P = 0.75) and telomerase activity (P = 0.88). Subsequent examination of cell-sorted CD38(+) and CD38(-) sub-populations from paired peripheral blood and bone marrow samples taken on the same day showed no significant difference in CD38, Ki-67, TERT expression or telomere lengths, indicating that these chronic lymphocytic leukaemia cells were derived from a single pool trafficking between these two compartments. Taken together, our data show that chronic lymphocytic leukaemia cells derived from bimodal patients all have extensive proliferative histories and have undergone a similar number of cell divisions that is mirrored by the episodic expression of CD38.
引用
收藏
页码:595 / 605
页数:11
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